1. Immunology/Inflammation
  2. Toll-like Receptor (TLR)
  3. Lipopolysaccharides, from S. enterica serotype typhimurium

Lipopolysaccharides, from S. enterica serotype typhimurium  (Synonyms: LPS, from Salmonella enterica (Serotype typhimurium))

目录号: HY-D1056C3
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Lipopolysaccharides, from S. enterica (Salmonella enterica) serotype typhimurium 是来源于血清型沙门氏菌 (S. enterica) 的脂多糖内毒素和 TLR4 激活剂,是 S 型 LPS。Lipopolysaccharides, from S. enterica 具有典型的 3 部分结构:O 抗原、核心寡糖和脂质 A。Lipopolysaccharides, from S. enterica serotype typhimurium 可调控细菌在树突状细胞(DC)中的命运,决定 DC 细胞对细菌的摄取、降解和免疫功能的激活。
建议配制 ≥2 mg/mL 母液,并保证充分混合溶解。由于 LPS 具有吸附特性,分装保存时需使用低吸附离心管

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Lipopolysaccharides, from S. enterica serotype typhimurium Chemical Structure

Lipopolysaccharides, from S. enterica serotype typhimurium Chemical Structure

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查看 Toll-like Receptor (TLR) 亚型特异性产品:

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Lipopolysaccharides, from S. enterica (Salmonella enterica) serotype typhimurium are lipopolysaccharide endotoxins and TLR4 activators derived from serotype typhimurium of Salmonella enterica, and are classified as S-type LPS. Lipopolysaccharides, from S. enterica exhibit a typical three-part structure: O-antigen, core oligosaccharide, and lipid A. Lipopolysaccharides, from S. enterica serotype typhimurium can modulate the fate of bacteria in dendritic cells (DC), determining the uptake, degradation, and activation of immune functions by DC cells against the bacteria[1][2][3][4].
It is recommended to prepare a stock solution of ≥2 mg/mL and ensure that it is fully mixed and dissolved. Due to the adsorption characteristics of LPS, low adsorption centrifuge tubes should be used for aliquoting and storage.

IC50 & Target

TLR-4[2]

体外研究
(In Vitro)

Note:
1. 为了保持 LPS 的完整性,建议将 LPS 溶液存放在硅烷化容器中。因为 LPS 可以粘附在塑料和某些类型的玻璃上,尤其是在浓度低于 0.1 mg/mL 时。如果 LPS 浓度超过 1 mg/mL,这种吸附作用相对较小,建议配制 ≥2 mg/mL 母液,并充分涡旋震荡 10 分钟以上,必要时辅助超声,保证充分混合溶解。如果使用玻璃容器,请确保使用前充分混合至少 30 分钟,以重新溶解任何吸附在管壁的 LPS。
2. LPS 是一种能在溶液中形成不同尺寸胶束的分子,分子量不固定,储备液及工作液可直接配制成质量浓度 (mg/mL, μg/mL, etc.)
3. LPS 溶于水或 PBS 后可能会观察到均匀的浑浊溶液,如需过滤除菌,请勿直接过滤储备液,建议稀释成工作液之后用 0.22 μm 的滤膜过滤除菌

Lipopolysaccharides, from S. enterica serotype typhimurium 是不同于普通沙门氏菌属分泌 LPS 的脂多糖。普通沙门氏菌的 LPS 只有一种野生型核心寡糖结构(血清型鼠伤寒沙门氏菌中的 Ra 核心),而血清型 Lipopolysaccharides, from S. enterica serotype typhimurium 与 Ra 核心在外部核心的单个末端侧枝上有所不同,可能决定了不同的免疫原性[1]S. enterica serotype typhimurium 拥有两种 Wzz 蛋白:WzzST (使 O 抗原的模态范围为 16-35 个 RU) 和 WzzfepE (使模态值超过 100 个 RU) (RU:糖重复单元)。而 Wzz 蛋白调控 Lipopolysaccharides 中 O 抗原的长度,附着于脂质 A 的 RU 数量由 Wzz 蛋白决定的模态值决定。突变对应的蛋白编码基因会导致形成短链 O 抗原链及细菌致病性显著降低[2]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

性状

固体

颜色

White to off-white

中文名称

脂多糖,来源于肠沙门氏菌肠道血清型

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

纯度 & 产品资料
参考文献

Lipopolysaccharides, from S. enterica serotype typhimurium 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Lipopolysaccharides, from S. enterica serotype typhimurium
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HY-D1056C3
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