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  3. Manganese chloride (tetrahydrate), molecular biology grade,≥99.0% (KT)

Manganese chloride (tetrahydrate), molecular biology grade,≥99.0% (KT)  (Synonyms: Manganese(Ⅱ) chloride (tetrahydrate), molecular biology grade,≥99.0% (KT))

目录号: HY-109521A 纯度: 99.08%
COA 产品使用指南 技术支持

Manganese chloride (tetrahydrate), molecular biology grade,≥99.0% (KT) 是一种可口服且能通过血脑屏障的化合物。Manganese chloride (tetrahydrate), molecular biology grade,≥99.0% (KT) 影响细胞内的多种酶活性如调节组蛋白乙酰转移酶 (HAT) 和组蛋白去乙酰化酶 (HDAC) 的活性,进而影响基因表达。Manganese chloride (tetrahydrate), molecular biology grade,≥99.0% (KT) 具有神经毒性、胚胎毒性、生殖毒性等多种活性,目前主要用于神经退行性疾病和毒理研究。

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Manganese chloride (tetrahydrate), molecular biology grade,≥99.0% (KT) Chemical Structure

Manganese chloride (tetrahydrate), molecular biology grade,≥99.0% (KT) Chemical Structure

CAS No. : 13446-34-9

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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Manganese chloride (tetrahydrate), molecular biology grade,≥99.0% (KT) is an orally active and a blood-brain barrier penetrant compound. It affects the activities of multiple enzymes in cells, such as regulating the activities of histone acetyltransferase (HAT) and histone deacetylase (HDAC), thereby affecting gene expression. It has multiple activities such as neurotoxicity, embryotoxicity, and reproductive toxicity. It is currently mainly used in neurodegenerative diseases and toxicology research.

体外研究
(In Vitro)

Manganese chloride tetrahydrate, molecular biology grade,≥99.0% (KT) (15-25 μM) 在培养的人淋巴细胞中具有细胞毒性,导致染色体畸变和 DNA 损伤[1]
Manganese chloride (300 μM;3-24 h) tetrahydrate, molecular biology grade,≥99.0% (KT) 在 PC12 细胞和 SH - SY5Y 细胞中,可显著抑制组蛋白 H3 和 H4 的乙酰化,改变 HAT 和 HDAC 的活性及表达水平[2]
Manganese chloride (0.1-10 mM; 5 days) tetrahydrate, molecular biology grade,≥99.0% (KT) 在小鼠胚胎肢芽细胞微团中,降低细胞活力,抑制细胞分化为软骨结节[3]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[2]

Cell Line: PC12 cells
Concentration: 300 μM
Incubation Time: 3 h, 6 h, 12 h, 24 h
Result: Reduced the acetylation of histone H3 and H4. Increased the activity of HDAC and Decreased that of HAT.
体内研究
(In Vivo)

Manganese chloride (1000-8000 mg/L;经饮水摄入,口服;持续 12 周) tetrahydrate, molecular biology grade, ≥99.0% (KT) 在 Swiss 小鼠中,会降低雄性小鼠生育能力,减少雌性小鼠的着床位点和活胎数量[4]
Manganese chloride (0-100 mg/kg;皮下注射;单剂量或多剂量;1 天或 7 天) tetrahydrate, molecular biology grade, ≥99.0% (KT) 在 C57BL/6 小鼠中,可使基底神经节锰浓度显著增加,导致水平运动减少[5]
Manganese chloride (0-50 mg/kg;口服灌胃;每天 1 次;21 天) tetrahydrate, molecular biology grade, ≥99.0% (KT) 在新生和成年 CD 大鼠中,高剂量会使新生大鼠体重增长减少、声学惊吓反应幅度增加,成年大鼠纹状体、小脑和脑残留锰浓度增加[6]
Manganese chloride (16 mg/kg/day;静脉输注;每天 1 次;4 天) tetrahydrate, molecular biology grade, ≥99.0% (KT) 在雄性比格犬中,导致动物死亡或濒死,出现严重肝毒性、低血压和反射性心动过速[7]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 male retired breeder mice (7-9 months old), model for studying manganese-induced neurotoxicity and its relation to Parkinson's disease[5]
Dosage: 50, 100 mg/kg (dissolved in distilled water)
Administration: Subcutaneous injection, single-dose or three-dose (for 7-day survival time), 1 day or 7 days
Result: Increased striatal manganese concentration by 105% in 1 day at a single 100 mg/kg dose. Increased striatal manganese concentration by 421% and 647% in 1 day at multiple doses of 50 and 100 mg/kg, respectively. Decreased horizontal movement at 50, 100 mg/kg (single-dose) and 100 mg/kg (multiple-dose).
Animal Model: Male beagle dogs (about 8-month-old), model for evaluating acute toxicologic potential of manganese chloride[7]
Dosage: 16 mg/kg/day (prepared as a 2 mg/mL solution in 0.9% sodium chloride for injection)
Administration: Intravenous infusion, once a day, for 4 days
Result: Showed clinical signs of toxicity such as loss of appetite, reduction in blood pressure with reflex tachycardia, and a marked increase in liver enzymes. Confirmed severe hepatotoxicity by gross and histopathologic evaluations.
分子量

197.91

Formula

Cl2H8MnO4

CAS 号
性状

固体

颜色

Off-white to pink

中文名称

氯化锰四水合物

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据
细胞实验: 

H2O 中的溶解度 : 1666.67 mg/mL (8421.35 mM; 超声助溶 (<60°C))

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 5.0528 mL 25.2640 mL 50.5280 mL
5 mM 1.0106 mL 5.0528 mL 10.1056 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

* 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳,请具体参考实测 水 / PBS / Saline 中的溶解度数据。
您所需的储备液浓度超过该产品的实测溶解度,如有需要,请与 MCE 中国技术支持联系。
纯度 & 产品资料

纯度: 99.08%

参考文献

Manganese chloride (tetrahydrate), molecular biology grade,≥99.0% (KT) 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 5.0528 mL 25.2640 mL 50.5280 mL 126.3200 mL
5 mM 1.0106 mL 5.0528 mL 10.1056 mL 25.2640 mL
10 mM 0.5053 mL 2.5264 mL 5.0528 mL 12.6320 mL
15 mM 0.3369 mL 1.6843 mL 3.3685 mL 8.4213 mL
20 mM 0.2526 mL 1.2632 mL 2.5264 mL 6.3160 mL
25 mM 0.2021 mL 1.0106 mL 2.0211 mL 5.0528 mL
30 mM 0.1684 mL 0.8421 mL 1.6843 mL 4.2107 mL
40 mM 0.1263 mL 0.6316 mL 1.2632 mL 3.1580 mL
50 mM 0.1011 mL 0.5053 mL 1.0106 mL 2.5264 mL
60 mM 0.0842 mL 0.4211 mL 0.8421 mL 2.1053 mL
80 mM 0.0632 mL 0.3158 mL 0.6316 mL 1.5790 mL
100 mM 0.0505 mL 0.2526 mL 0.5053 mL 1.2632 mL

* 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Manganese chloride (tetrahydrate), molecular biology grade,≥99.0% (KT)
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HY-109521A
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