2. Academic Validation
  3. Integration of Transcriptomics and Metabolomics for Pepper (Capsicum annuum L.) in Response to Heat Stress

Integration of Transcriptomics and Metabolomics for Pepper (Capsicum annuum L.) in Response to Heat Stress

  • Int J Mol Sci. 2019 Oct 11;20(20):5042. doi: 10.3390/ijms20205042.
Jing Wang 1,  Junheng Lv 2,  Zhoubin Liu 3,  Yuhua Liu 4,  Jingshuang Song 5,  Yanqing Ma 6,  Lijun Ou 7,  Xilu Zhang 8,  Chengliang Liang 9,  Fei Wang 10,  Niran Juntawong 11,  Chunhai Jiao 12,  Wenchao Chen 13,  Xuexiao Zou 14 15
Affiliations

Affiliations

  • 1 Longping Branch, Graduate School of Hunan University, Changsha 410125, China. wangjinghnu@hnu.edu.cn.
  • 2 Longping Branch, Graduate School of Hunan University, Changsha 410125, China. junhenglv@hnu.edu.cn.
  • 3 Longping Branch, Graduate School of Hunan University, Changsha 410125, China. hnliuzhoubin@163.com.
  • 4 Longping Branch, Graduate School of Hunan University, Changsha 410125, China. liuyuhua@hnu.edu.cn.
  • 5 Longping Branch, Graduate School of Hunan University, Changsha 410125, China. sjs122912@163.com.
  • 6 Hunan Vegetable Research Institute, Changsha 410125, China. yanqingmahn@163.com.
  • 7 College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410125, China. ou9572@126.com.
  • 8 Hunan Vegetable Research Institute, Changsha 410125, China. ytuwangjing@163.com.
  • 9 Hunan Vegetable Research Institute, Changsha 410125, China. chenwenchao2019@163.com.
  • 10 Hubei Academy of Agricultural Sciences, Wuhan 430064, China. raymond@163.com.
  • 11 Department of Botany, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand. niranjuntawong@yahoo.com.
  • 12 Hubei Academy of Agricultural Sciences, Wuhan 430064, China. Jiaoch@Hotmail.com.
  • 13 Hunan Vegetable Research Institute, Changsha 410125, China. wencc1974@163.com.
  • 14 Longping Branch, Graduate School of Hunan University, Changsha 410125, China. zouxuexiao428@163.com.
  • 15 College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410125, China. zouxuexiao428@163.com.
PMID: 31614571 DOI: 10.3390/ijms20205042
Abstract

Heat stress (HS), caused by extremely high temperatures, is one of the most severe forms of abiotic stress in pepper. In the present study, we studied the transcriptome and metabolome of a heat-tolerant cultivar (17CL30) and a heat-sensitive cultivar (05S180) under HS. Briefly, we identified 5754 and 5756 differentially expressed genes (DEGs) in 17CL30 and 05S180, respectively. Moreover, we also identified 94 and 108 differentially accumulated metabolites (DAMs) in 17CL30 and 05S180, respectively. Interestingly, there were many common HS-responsive genes (approximately 30%) in both pepper cultivars, despite the expression patterns of these HS-responsive genes being different in both cultivars. Notably, the expression changes of the most common HS-responsive genes were typically much more significant in 17CL30, which might explain why 17CL30 was more heat tolerant. Similar results were also obtained from metabolome data, especially Amino acids, organic acids, Flavonoids, and sugars. The changes in numerous genes and metabolites emphasized the complex response mechanisms involved in HS in pepper. Collectively, our study suggested that the glutathione metabolic pathway played a critical role in pepper response to HS and the higher accumulation ability of related genes and metabolites might be one of the primary reasons contributing to the heat resistance.

Keywords

glutathione; heat shock protein; heat shock transcription factors; metabolome; transcriptome.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z