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  2. Lack of cytotoxic and genotoxic effects of mPEG-silane coated iron(III) oxide nanoparticles doped with magnesium despite cellular uptake in cancerous and noncancerous lung cells

Lack of cytotoxic and genotoxic effects of mPEG-silane coated iron(III) oxide nanoparticles doped with magnesium despite cellular uptake in cancerous and noncancerous lung cells

  • Toxicol In Vitro. 2024 Aug:99:105850. doi: 10.1016/j.tiv.2024.105850.
Malgorzata Sikorska 1 Monika Ruzycka-Ayoush 2 Ivan Rios-Mondragon 3 Eleonora Marta Longhin 4 Sylwia Meczynska-Wielgosz 5 Maria Wojewodzka 5 Agata Kowalczyk 6 Artur Kasprzak 7 Julita Nowakowska 8 Kamil Sobczak 9 Magdalena Muszynska 10 Mihaela Roxana Cimpan 3 Elise Runden-Pran 4 Sergey Shaposhnikov 11 Marcin Kruszewski 12 Maria Dusinska 4 Anna M Nowicka 6 Ireneusz P Grudzinski 2
Affiliations

Affiliations

  • 1 Department of Toxicology and Food Science, Faculty of Pharmacy, Medical University of Warsaw, Banacha Str. 1, PL-02-097 Warsaw, Poland. Electronic address: malgorzata.sikorska@wum.edu.pl.
  • 2 Department of Toxicology and Food Science, Faculty of Pharmacy, Medical University of Warsaw, Banacha Str. 1, PL-02-097 Warsaw, Poland.
  • 3 Department of Clinical Dentistry, Faculty of Medicine, University of Bergen, Årstadveien. 19, Bergen 5009, Norway.
  • 4 Health Effects Laboratory, Department of Environmental Chemistry, Norwegian Institute for Air Research, 2007 Kjeller, Norway.
  • 5 Centre for Radiobiology and Biological Dosimetry, Institute of Nuclear Chemistry and Technology, Dorodna Str. 16, PL-03-195, Warsaw, Poland.
  • 6 Department of Inorganic and Analytical Chemistry, Faculty of Chemistry, University of Warsaw, Pasteura Str. 1, PL-02-093 Warsaw, Poland.
  • 7 Department of Organic Chemistry, Faculty of Chemistry, Warsaw University of Technology, Noakowskiego Str. 3, PL-00-664 Warsaw, Poland.
  • 8 Laboratory of Electron and Confocal Microscopy, Faculty of Biology, University of Warsaw, Miecznikowa Str.1, PL-02-096 Warsaw, Poland.
  • 9 Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, Zwirki i Wigury 101 Str., PL 02-089 Warsaw, Poland.
  • 10 Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, Zwirki i Wigury 101 Str., PL 02-089 Warsaw, Poland; Pro-Environment Poland Sp. z o. o., Zwirki i Wigury Str. 101, PL 02-098 Warsaw, Poland.
  • 11 Norgenotech AS, Gaustadalleen Str. 21, Oslo 0349, Norway.
  • 12 Centre for Radiobiology and Biological Dosimetry, Institute of Nuclear Chemistry and Technology, Dorodna Str. 16, PL-03-195, Warsaw, Poland; Department of Medical Biology and Translational Research, Institute of Rural Health,Jaczewskiego Str. 2, PL-20-090 Lublin, Poland.
Abstract

Cytotoxic and genotoxic effects of novel mPEG-silane coated iron(III) oxide nanoparticles doped with magnesium (Mg0.1-γ-Fe2O3(mPEG-silane)0.5) have been investigated on human adenocarcinomic alveolar basal epithelial (A549) and human normal bronchial epithelial (BEAS-2B) cells. In the studies several molecular and cellular targets addressing to cell membrane, cytoplasm organelles and nucleus components were served as toxicological endpoints. The as-synthesized nanoparticles were found to be stable in the Cell Culture media and were examined for different concentration and exposure times. No cytotoxicity of the tested nanoparticles was found although these nanoparticles slightly increased Reactive Oxygen Species in both cell types studied. Mg0.1-γ-Fe2O3(mPEG-silane)0.5 nanoparticles did not produce any DNA strand breaks and oxidative DNA damages in A549 and BEAS-2B cells. Different concentration of Mg0.1-γ-Fe2O3(mPEG-silane)0.5 nanoparticles and different incubation time did not affect cell migration. The lung Cancer cells' uptake of the nanoparticles was more effective than in normal lung cells. Altogether, the results evidence that mPEG-silane coated iron(III) oxide nanoparticles doped with magnesium do not elucidate any deleterious effects on human normal and cancerous lung cells despite cellular uptake of these nanoparticles. Therefore, it seems reasonable to conclude that these novel biocompatible nanoparticles are promising candidates for further development towards medical applications.

Keywords

Cytotoxicity; Genotoxicity; Lung cells; Magnesium; iron oxide nanoparticles; mPEG-silane.

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