2. Immunology/Inflammation Apoptosis
  3. Toll-like Receptor (TLR) Apoptosis
  4. Poly (I:C):Kanamycin (1:1) (sodium)

Poly (I:C):Kanamycin (1:1) (sodium) 

目录号: HY-135748A
产品使用指南 技术支持

Poly (I:C):Kanamycin (1:1) sodium 是 Poly (I:C) (HY-135748) 和 Kanamycin (HY-16566) 的等比例复合物。Poly(I:C) sodium 是双链 RNA 的合成类似物，是一种 TLR3 和视黄酸诱导型基因 I 受体 (RIG-I 和 MDA5) 的激动剂。Poly(I:C) sodium 可以用作疫苗佐剂，增强先天性和适应性免疫反应，并诱导癌细胞凋亡。Kanamycin 是一种具有口服活性的抗菌剂（革兰氏阴性/阳性细菌），可抑制易位并通过与 70 S 核糖体亚基结合引起错误编码。Kanamycin 对结核分枝杆菌（敏感和耐药）和肺炎克雷伯菌均表现出良好的抑制活性，可用于结核病和肺炎的研究。

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Poly (I:C):Kanamycin (1:1) (sodium) Chemical Structure

Poly (I:C):Kanamycin (1:1) (sodium) Chemical Structure

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规格 价格 是否有货 数量
1 mg ￥560
In-stock
5 mg ￥1700
In-stock
10 mg ￥2700
In-stock
25 mg ￥5400
In-stock
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100 mg   询价  

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Customer Review

Other Forms of Poly (I:C):Kanamycin (1:1) (sodium):

Poly (I:C):Kanamycin (1:1) (sodium) 相关抗体

Top Publications Citing Use of Products

    Poly (I:C):Kanamycin (1:1) (sodium) purchased from MCE. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    BECN1 knockout HeLa cells were transfected with pCAGGS, HA-8A or HA-NSs for 24 h, and then transfected with poly(I:C) for 24 h. IFNB, IFIT1 and CXCL10 mRNA levels were analyzed with RT-qPCR.

    Poly (I:C):Kanamycin (1:1) (sodium) purchased from MCE. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    HeLa cells were transfected with pCAGGS, HA-8A or HA-NSs at a dose of 2 μg for 24 h, and then transfected with poly(I:C) for 24 h. IFNB, IFIT1 and CXCL10 mRNA levels were analyzed with RT-qPCR.

    Poly (I:C):Kanamycin (1:1) (sodium) purchased from MCE. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    HEK293T cells were transfected with HA-8A or HA-NSs at a dose of 2 μg for 24 h, and then transfected with poly(I:C) for 24 h. Co-IP with HA antibodies and evaluation of LC3, TBK1 and TRIM25 with western blot using IgG as control.

    Poly (I:C):Kanamycin (1:1) (sodium) purchased from MCE. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    HEK293T cells were transfected with pCAGGS, HA-NSs and HA-8A, HA-PxxP, HA-DWP, or HA-WxxL at a dose of 2 μg for 24 h, and then transfected with poly(I:C) for 24 h. IFNB and IFIT1 mRNA levels were analyzed with RT-qPCR.

    Poly (I:C):Kanamycin (1:1) (sodium) purchased from MCE. Usage Cited in: Chem Eng J. 2021 Aug 15;418:129392.

    Immunofluorescence staining photographs of lung sections. Scale bars, 100 μm. (I: PBS, II: Poly(I:C), III: PL-Poly(I:C), IV: NL-Poly(I:C), V: PL-Poly(I:C)/RBD, VI: NL-Poly(I:C)/RBD).

    查看 Toll-like Receptor (TLR) 亚型特异性产品:

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    TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Poly (I:C):Kanamycin (1:1) sodium is an isometric complex of Poly (I:C) (HY-135748) and Kanamycin (HY-16566). Poly(I:C) sodium, a synthetic analog of double-stranded RNA, is a TLR3 and retinoic acid-inducible gene I receptor (RIG-I and b>MDA5) agonist. Poly(I:C) sodium can be used as a vaccine adjuvant to enhance innate and adaptive immune responses and induce apoptosis in cancer cells[1][2]. Kanamycin is an orally active antibacterial agent (Gram-negative/positive bacteria) that inhibits translocation and causes miscoding by binding to the 70S ribosomal subunit. Kanamycin shows good inhibitory activity against Mycobacterium tuberculosis (susceptible and drug-resistant) and Klebsiella pneumoniae, and can be used in the research of tuberculosis and pneumonia[3][4][5][6].

    IC50 & Target

    TLR3[2], RIG-I[2], MDA5[2], apoptosis[2]
    体外研究
    (In Vitro)

    Poly (I:C) sodium（20 ng/mL；24 小时；WM793、WM278、WM239A、WM9 和 1205Lu 细胞）处理细胞后，可在 24 小时内显着降低存活率，从对照的 100% 降至 20%-50%[1].
    Poly (I:C) sodium（200 ng/mL；24 小时；1205Lu 细胞）处理诱导 1205Lu 细胞凋亡[1]。
    Polyinosinic-polycytidylic acid（3 ng/mL；24 小时；1205Lu 细胞）处理诱导黑色素瘤细胞中的 IFN-β 表达。 RIG-I 和 MDA-5 的沉默证实 Poly (I:C) sodium 诱导 IFN-β 分别需要 RIG-I 和 MDA-5，并且需要 IPS-1[1]。< br/> Poly (I:C) sodium（5 ng/mL；24 小时；1205Lu 细胞）处理揭示黑素瘤细胞中 caspase-9 和 caspase-8 的活性亚基[1]。

    Kanamycin (0.1-100 μg/mL; 2 周) 在体外对多种分枝杆菌菌株表现出良好的抗菌活性 (MIC=1-5 μg/mL)[3]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Poly (I:C):Kanamycin (1:1) (sodium) 相关抗体:
    体内研究
    (In Vivo)

    Poly (I:C) sodium 可抑制注射 1205Lu 细胞的 NOD/SCID 免疫缺陷小鼠的肿瘤生长。在用 Poly (I:C) sodium 处理的小鼠中，人类 DNA 的水平降低了 50%[1]。

    Kanamycin（2、4 mg/kg；皮下注射；每天一次，每周 6 次，持续 3 周）抑制小鼠肺和脾脏中牛结核杆菌的生长[3]。
    Kanamycin（1.25、5 mg/kg；皮下注射；感染后 3 小时，单次注射）抑制肺炎克雷伯菌 DT-S 在小鼠肺、气管和血液中的增殖，并且与给药剂量成比例，并且还会增加小鼠的存活率[4]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    性状

    固体

    颜色

    White to off-white

    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    -20°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    溶解性数据
    细胞实验： 

    H2O 中的溶解度 : 50 mg/mL (超声助溶)

    DMSO 中的溶解度 : < 1 mg/mL (insoluble or slightly soluble)

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
    您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
    纯度 & 产品资料
    参考文献

    Poly (I:C):Kanamycin (1:1) (sodium) 相关分类

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Poly (I:C):Kanamycin (1:1) (sodium)Toll-like Receptor (TLR)ApoptosisTLR3RIG-IMDA5immunotherapytumormicroenvironment apoptosisdsRNAproperannealingdouble-strandedRNAInhibitorinhibitorinhibit

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