2. Immunology/Inflammation Apoptosis Cell Cycle/DNA Damage Metabolic Enzyme/Protease
  3. Toll-like Receptor (TLR) PKD HSP Bcl-2 Family Interleukin Related Apoptosis
  4. Polyinosinic-polycytidylic acid

Polyinosinic-polycytidylic acid  (Synonyms: Poly(I:C))

目录号: HY-107202 含量: 99.4%
COA 产品使用指南 技术支持

Polyinosinic-polycytidylic acid (Poly(I:C)) 是双链 RNA 的合成类似物，是一种 TLR3 和视黄酸诱导型基因 I 受体 (RIG-I 和 MDA5) 的激动剂。Polyinosinic-polycytidylic acid 可以用作疫苗佐剂，以增强先天性和适应性免疫反应，并改变肿瘤的微环境，还可以直接触发癌细胞发生凋亡 (apoptosis)。

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CAS No. : 24939-03-5

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25 mg ￥1705
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Polyinosinic-polycytidylic acid 相关抗体

Top Publications Citing Use of Products

    Polyinosinic-polycytidylic acid purchased from MCE. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    BECN1 knockout HeLa cells were transfected with pCAGGS, HA-8A or HA-NSs for 24 h, and then transfected with poly(I:C) for 24 h. IFNB, IFIT1 and CXCL10 mRNA levels were analyzed with RT-qPCR.

    Polyinosinic-polycytidylic acid purchased from MCE. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    HeLa cells were transfected with pCAGGS, HA-8A or HA-NSs at a dose of 2 μg for 24 h, and then transfected with poly(I:C) for 24 h. IFNB, IFIT1 and CXCL10 mRNA levels were analyzed with RT-qPCR.

    Polyinosinic-polycytidylic acid purchased from MCE. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    HEK293T cells were transfected with HA-8A or HA-NSs at a dose of 2 μg for 24 h, and then transfected with poly(I:C) for 24 h. Co-IP with HA antibodies and evaluation of LC3, TBK1 and TRIM25 with western blot using IgG as control.

    Polyinosinic-polycytidylic acid purchased from MCE. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    HEK293T cells were transfected with pCAGGS, HA-NSs and HA-8A, HA-PxxP, HA-DWP, or HA-WxxL at a dose of 2 μg for 24 h, and then transfected with poly(I:C) for 24 h. IFNB and IFIT1 mRNA levels were analyzed with RT-qPCR.

    Polyinosinic-polycytidylic acid purchased from MCE. Usage Cited in: Chem Eng J. 2021 Aug 15;418:129392.

    Immunofluorescence staining photographs of lung sections. Scale bars, 100 μm. (I: PBS, II: Poly(I:C), III: PL-Poly(I:C), IV: NL-Poly(I:C), V: PL-Poly(I:C)/RBD, VI: NL-Poly(I:C)/RBD).

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    IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22

    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Polyinosinic-polycytidylic acid (Poly(I:C)) is a synthetic analog of double-stranded RNA and an agonist of toll-like receptor 3 (TLR3) and retinoic acid inducible gene I (RIG-I)-like receptors (RIG-I and MDA5). Polyinosinic-polycytidylic acid can be used as a vaccine adjuvant to enhance innate and adaptive immune responses, and to alter the tumor microenvironment. Polyinosinic-polycytidylic acid can directly trigger cancer cells to undergo apoptosis[1][2].

    IC50 & Target

    Bcl-2

     

    Bax

     

    IL-17A

     

    IL-13

     

    HSP70

     

    体外研究
    (In Vitro)

    Polyinosinic-polycytidylic acid (0.5-5 μg/mL, 3-24 h) 呈现剂量和时间依赖性增加诱导永生化气道上皮细胞的细胞旁通透性[4]。
    Polyinosinic-polycytidylic acid (5 μg/mL, 24 h) 对 16HBE14o- 细胞没有细胞毒性[4]。
    Polyinosinic-polycytidylic acid (5 μg/mL, 6 h) 在 16HBE14o- 细胞中诱导上皮顶端连接复合体和紧密连接的破坏[4]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Polyinosinic-polycytidylic acid 相关抗体:

    Cell Cytotoxicity Assay[4]

    Cell Line: 16HBE14o- cells
    Concentration: 5 μg/mL
    Incubation Time: 24 h
    Result: Did not lead to significant accumulation of LDH in cell-culture medium.
    体内研究
    (In Vivo)

    Polyinosinic-polycytidylic acid (2.5-10 mg/mL, Stereotaxic injection, single dose) 在脑黑质和背外侧纹状体中诱导持续的炎症反应[2]。
    Polyinosinic-polycytidylic acid (10 μg/mouse, Intraperitoneal injection, single dose) 降低小鼠的肺肿瘤生长[3]。
    Polyinosinic-polycytidylic acid (1.25 mg/kg, Intraperitoneal injection, single dose) 在 MCAO 模型小鼠中通过 TLR3 下调TLR4/MyD88 信号通路发挥脑缺血再灌注损伤的治疗作用[5]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: lung tumor-bearing mice[3]
    Dosage: 10 μg/mouse
    Administration: Intraperitoneal injection (i.p.)
    Result: Induced a significant decrease in the growth of pulmonary metastases in tumor-bearing mice.
    Reduced the amount of lung foci to ≈ 40%.
    Significantly increased BAL fluid cell numbers.
    Increased the level of INF-γ and IL-17A, decreased the levels of IL-13.
    Increased TLR3 expression.
    Animal Model: Middle cerebral artery occlusion (MCAO) model mice[5]
    Dosage: 1.25 mg/kg
    Administration: Intraperitoneal injection (i.p.)
    Result: Reduced focal cerebral I/R injury.
    Increased the expression of Bcl2, Hsp27, and Hsp70, decreased Bax expression, and reduced cellular degeneration and apoptosis.
    Protected against cerebral ischemia and conferred protection against cerebral I/R injury through the downregulation of TLR4 signaling via TLR3.
    Formula

    (C10H13N4O8P)x.(C9H14N3O8P)x
    CAS 号
    性状

    固体

    颜色

    White to off-white

    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式
    Powder -20°C 3 years
    In solvent -80°C 6 months
    -20°C 1 month
    溶解性数据
    细胞实验： 

    H2O 中的溶解度 : 50 mg/mL (超声助溶)

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： PBS

      Solubility: 100 mg/mL; 澄清溶液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
    您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
    纯度 & 产品资料

    纯度: 99.4%

    COA (259 KB)

    产品使用指南 (1538 KB)
    参考文献

    Polyinosinic-polycytidylic acid 相关分类

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Polyinosinic-polycytidylic acid24939-03-5Poly(I:C)Toll-like Receptor (TLR)PKDHSPBcl-2 FamilyInterleukin RelatedApoptosisProtein kinase DHeat shock proteinsIL16HBE14o- cellsagonistlung tumor-bearing miceMiddle cerebral artery occlusion (MCAO) model miceInhibitorinhibitorinhibit

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