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  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. Acetylcholinesterase/ACHE 蛋白, Human (Active, HEK293, His)

Acetylcholinesterase/ACHE 蛋白, Human (Active, HEK293, His)

目录号: HY-P704343
产品使用指南 技术支持

乙酰胆碱酯酶 (ACHE) 蛋白在神经传递中至关重要，可快速水解突触间隙中的乙酰胆碱，从而终止神经肌肉接头处的信号转导。除了神经递质调节之外，ACHE 还与神经元凋亡有关，这表明 ACHE 在与程序性细胞死亡相关的细胞过程中发挥着更广泛的作用。Acetylcholinesterase/ACHE 蛋白, Human (Active, HEK293, His) 是重组的 Acetylcholinesterase/ACHE 蛋白，由 HEK293 表达，带有 C-His 标签。

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100 μg   询价  

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描述

Acetylcholinesterase (ACHE) protein is pivotal in neurotransmission, rapidly hydrolyzing acetylcholine in the synaptic cleft to terminate signal transduction at the neuromuscular junction. Beyond neurotransmitter regulation, ACHE is implicated in neuronal apoptosis, indicating a broader role in cellular processes associated with programmed cell death. Acetylcholinesterase/ACHE Protein, Human (Active, HEK293, His) is the recombinant human-derived Acetylcholinesterase/ACHE protein, expressed by HEK293, with C-His labeled tag.
研究背景

乙酰胆碱酯酶（ACHE）蛋白通过快速水解突触间隙中的乙酰胆碱神经递质，从而促进神经肌肉接头信号转导的终止，在神经传递中发挥着至关重要的作用。除了其在神经递质调节中的基本功能之外，ACHE 还与神经元凋亡有关，这表明 ACHE 在与程序性细胞死亡相关的细胞过程中发挥着更广泛的作用。
种属

Human
表达系统

HEK293
标签

C-His
蛋白编号

P22303-1 (E32-L614)
基因 ID

43
蛋白结构
N-term
ACHE (E32-L614)
Accession # P22303
His
C-term
蛋白长度

Full Length of Isoform-1 Mature Protein
同用名
acetylcholinesterase (Yt blood group); Acetylcholinesterase; ACHE; apoptosis-related acetylcholinesterase; ARACHE; EC 3.1.1; EC 3.1.1.7; N-ACHE; Yt blood group; YT
预测分子量
66.5 kDa
分子量

Approximately 65-80 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.
糖基化修饰
Yes
纯度

Greater than 95% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Acetylcholinesterase/ACHE Protein, Human (Active, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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