Alkaline Phosphatase/ALPG 蛋白, Human (HEK293, His)

Alkaline Phosphatase/ALPG protein is an enzyme that hydrolyzes phosphomonoesters under alkaline conditions to release inorganic phosphate. Alkaline Phosphatase is involved in processes such as bone mineralization and intestinal phosphate absorption, and exhibits anti-inflammatory activity. Alkaline Phosphatase/ALPG protein, Human (HEK293, His), is a recombinant Alkaline Phosphatase/ALPG protein expressed in HEK293 cells and tagged with a C-terminal His tag^[1].

Alkaline Phosphatase/ALPG 蛋白是一种能够水解一系列磷酸盐化合物的酶。它具有通过其酶活性分解各种磷酸盐分子的能力。Alkaline Phosphatase 充当碱性磷酸酶，在碱性环境中催化磷酸酯的水解。其酶活性使其能够从核苷酸、蛋白质和磷脂等分子中裂解磷酸基团，使它们失去活性。这种多功能酶在各种生理过程中发挥着至关重要的作用，包括骨矿化、细胞信号传导和新陈代谢^[1]。

Alkaline Phosphatase 在人肾细胞、Henle 肠细胞等上皮细胞系中呈现组成型表达，而在 HeLa Henle、HeLa NIH 等细胞中无基础表达^[2]。

Alkaline Phosphatase (Human; 200 µg; 皮下注射; 2-4 次) 在大鼠关节炎模型中，可显著减少滑膜巨噬细胞浸润，并表现出全身抗炎作用，与 Methotrexate (HY-14519) 联用具有协同效应^[3]。

1.The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
2.Immobilized Human Alkaline Phosphatase (Germ type) , His Tag at 0.5 μg/mL (100 μl/well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC₅₀ of ≤8 ng/mL determined by ELISA.

Human

HEK293

C-His

P10696 (I20-D503)

251  [NCBI]

Full Length of Mature Protein

58-68 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from 0.22 μm filtered solution in 25 mM Tris, 100 mM NaCl, 1 mM MgCl₂ (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Sharma U, et al. Alkaline phosphatase: an overview. Indian J Clin Biochem. 2014 Jul;29(3):269-78.  [Content Brief]

  [2]. COX RP, et al. Alkaline phosphatase content and the effects of prednisolone on mammalian cells in culture. J Gen Physiol. 1962 Jan;45(3):439-85.  [Content Brief]

  [3]. Chandrupatla DMSH, et al. Prophylactic and therapeutic activity of alkaline phosphatase in arthritic rats: single-agent effects of alkaline phosphatase and synergistic effects in combination with methotrexate. Transl Res. 2018 Sep;199:24-38.  [Content Brief]