2. 重组蛋白
  3. Enzymes & Regulators
  4. Hydrolases (EC 3) Phosphatase
  5. Alkaline Phosphatase
  6. Alkaline Phosphatase/ALPG 蛋白, Human (HEK293, His)

Alkaline Phosphatase/ALPG 蛋白, Human (HEK293, His)

目录号: HY-P700952
产品使用指南

碱性磷酸酶/ALPG 蛋白是一种酶，可水解磷酸盐化合物，分解各种分子。它充当碱性磷酸酶，裂解磷酸酯并使分子失活。这种多功能酶在骨矿化、细胞信号传导和新陈代谢等过程中至关重要。Alkaline Phosphatase/ALPG 蛋白, Human (HEK293, His) 是重组的 碱性磷酸酶/ALPG 蛋白，由 HEK293 表达，带有 C-His 标签。Alkaline Phosphatase/ALPG 蛋白, Human (HEK293, His) 全长 484 个氨基酸，分子量为 58-68 kDa。

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描述

The Alkaline Phosphatase/ALPG protein, an enzyme, hydrolyzes phosphate compounds, breaking down various molecules. It acts as an alkaline phosphatase, cleaving phosphate esters and rendering molecules inactive. This versatile enzyme is vital in processes like bone mineralization, cellular signaling, and metabolism. Alkaline Phosphatase/ALPG Protein, Human (HEK293, His) is the recombinant human-derived Alkaline Phosphatase/ALPG protein, expressed by HEK293, with C-His labeled tag. The total length of Alkaline Phosphatase/ALPG Protein, Human (HEK293, His) is 484 a.a., with molecular weight of 58-68 kDa.
研究背景

碱性磷酸酶/ALPG 蛋白是一种能够水解一系列磷酸盐化合物的酶。它具有通过其酶活性分解各种磷酸盐分子的能力。这种蛋白质充当碱性磷酸酶，在碱性环境中催化磷酸酯的水解。其酶活性使其能够从核苷酸、蛋白质和磷脂等分子中裂解磷酸基团，使它们失去活性。这种多功能酶在各种生理过程中发挥着至关重要的作用，包括骨矿化、细胞信号传导和新陈代谢。
种属

Human
表达系统

HEK293
标签

C-His
蛋白编号

P10696 (I20-D503)
基因 ID

251  [NCBI]
蛋白结构
N-term
ALPG (I20-D503)
Accession # P10696
His
C-term
同用名
Alkaline Phosphatase; ALP-1; Alkaline phosphatase, placental-like; GCAP; PLAP-like; ALPG; ALPPL; ALPPL2
分子量

58-68 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Alkaline Phosphatase/ALPG Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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