Animal-Free IL-1 beta 蛋白, Human (His)

IL-1 beta protein is a potent proinflammatory cytokine that plays multiple roles in immune responses. It induces inflammatory events including prostaglandin synthesis, neutrophil activation, and cytokine production. Animal-Free IL-1 beta Protein, Human (His) is the recombinant human-derived animal-FreeIL-1 beta protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-1 beta Protein, Human (His) is 153 a.a., with molecular weight of ~18.48 kDa.

IL-1 β 蛋白是一种有效的促炎细胞因子，因其在协调免疫反应中的多种作用而受到认可。IL-1β最初被认为是一种主要的内源性热原，可诱导一系列炎症事件，包括前列腺素合成、中性粒细胞流入和激活、T细胞和B细胞激活、细胞因子产生以及成纤维细胞增殖和胶原蛋白产生。它在免疫细胞分化中发挥着关键作用，促进 T 细胞的 Th17 分化，并与 IL-12 协同诱导辅助性 T 1 (Th1) 细胞合成 IFNG。此外，IL-1β 通过诱导 VEGF 产生，与 TNF 和 IL-6 协同作用，促进血管生成。值得注意的是，它在细胞焦亡下游炎症转导中发挥着关键作用，通过gasdermin-D (GSDMD) 孔特异性释放到细胞外环境中。在微生物感染的情况下，IL-1β充当皮肤中化脓性链球菌感染的传感器，被化脓性链球菌SpeB蛋白酶裂解和激活，导致炎症反应，从而在侵袭性皮肤感染期间抑制细菌生长。然而，SpeB 对 IL-1β 的裂解具有双重作用，通过破坏微生物群介导的定植抵抗来促进鼻咽链球菌感染。

1.Measure by its ability to induce proliferation in D10.G4.1 cells. The ED₅₀ for this effect is <10 pg/mL. The specific activity of recombinant human IL-1 beta is approximately >1.5 x 10⁸ IU/mg.
2.Measure by its ability to induce IL-8 secretion in HT29 cells. The ED₅₀ for this effect is ≤5.1 ng/mL.

Human

E. coli

C-His

P01584 (A117-S269)

3553  [NCBI]

MASAPVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS

Approximately 18.48 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a solution containing 1X PBS, pH 8.0.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.