Animal-Free IL-33 蛋白, Mouse (His)

IL-33 protein activates NF-kappa-B and MAPK signaling, inducing Th2 cell maturation and cytokine secretion. It activates mast cells, basophils, eosinophils, and natural killer cells and enhances macrophage polarization. Animal-Free IL-33 Protein, Mouse (His) is the recombinant mouse-derived animal-FreeIL-33 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-33 Protein, Mouse (His) is 158 a.a., with molecular weight of ~18.51 kDa.

IL-33 蛋白是一种细胞因子，与 IL1RL1/ST2 受体结合并通过其发出信号，激活靶细胞中的 NF-kappa-B 和 MAPK 信号通路。它参与 Th2 细胞的成熟，诱导 T 辅助细胞 2 型相关细胞因子的分泌。此外，IL-33 参与肥大细胞、嗜碱性粒细胞、嗜酸性粒细胞和自然杀伤细胞的激活，充当交替激活的巨噬细胞极化的增强剂。它可作为 Th2 细胞的化学引诱剂，并可起到“警报器”的作用，在组织损伤期间放大免疫反应。值得注意的是，它诱导 UCP2 依赖性线粒体快速重新布线，减少活性氧的产生，并保持持续产生衣康酸所需的克雷布斯循环的完整性，从而导致 GATA3 依赖性炎症解决替代激活巨噬细胞的分化。在静止内皮细胞中，未切割形式的 IL-33 组成型且大量表达，作为具有转录抑制特性的染色质相关核因子，可能隔离核 NF-kappaB/RELA 并降低其靶标的表达。这种形式在血管生成或促炎激活后迅速消失。

Measure by its ability to induce proliferation in D10.G4.1 cells. The ED₅₀ for this effect is <40 pg/mL. The specific activity of recombinant mouse IL-33 is >2 x 10⁶ IU/mg.

Mouse

E. coli

C-His

Q8BVZ5 (S109-I266)

77125  [NCBI]

MSIQGTSLLTQSPASLSTYNDQSVSFVLENGCYVINVDDSGKDQEQDQVLLRYYESPCPASQSGDGVDGKKLMVNMSPIKDTDIWLHANDKDYSVELQRGDVSPPEQAFFVLHKKSSDFVSFECKNLPGTYIGVKDNQLALVEEKDESCNNIMFKLSKI

Approximately 18.51 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 7.4.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.