Animal-Free IP-10/CRG-2/CXCL10 蛋白, Human (His)

The IP-10/CRG-2/CXCL10 protein is a pro-inflammatory cytokine involved in a variety of biological processes, including chemotaxis, immune cell activation, growth regulation, apoptosis, and vasostatic regulation. During viral infection, IP-10 crucially stimulates immune cell activation and migration to the site of infection. Animal-Free IP-10/CRG-2/CXCL10 Protein, Human (His) is the recombinant human-derived animal-FreeIP-10/CRG-2/CXCL10 protein, expressed by E. coli , with N-His labeled tag. The total length of Animal-Free IP-10/CRG-2/CXCL10 Protein, Human (His) is 77 a.a., with molecular weight of ~9.45 kDa.

IP-10 (CXCL10) 是一种促炎细胞因子，参与多种生物过程，包括外周免疫细胞的趋化性、分化和激活、细胞生长调节、细胞凋亡和血管抑制作用的调节。值得注意的是，在病毒感染期间，IP-10 通过刺激免疫细胞激活和迁移到感染部位而发挥关键作用。从机制上讲，CXCL10 与 CXCR3 受体的结合激活 G 蛋白介导的信号传导，导致下游磷脂酶 C 依赖性途径激活、细胞内钙生成增加和肌动蛋白重组。这种级联反应导致活化的 Th1 淋巴细胞募集到炎症部位。CXCL10/CXCR3 轴在神经元中也具有重要意义，它通过激活小胶质细胞（中枢神经系统的常驻巨噬细胞群）来响应脑损伤，并引导它们到达病变部位，这是神经元重组的关键要素。IP-10 以单体、二聚体和四聚体形式存在，并与 CXCR3 相互作用，特别是通过其 N 末端。

Measure by its ability to chemoattract BaF3 cells transfected with human CXCR3. The ED₅₀ for this effect is < 0.15 μg/mL.

Human

E. coli

N-His

P02778 (V22-P98)

3627  [NCBI]

VPLSRTVRCTCISISNQPVNPRSLEKLEIIPASQFCPRVEIIATMKKKGEKRCLNPESKAIKNLLKAVSKERSKRSP

Approximately 9.45 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 7.4.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.