1. 重组蛋白
  2. Others
  3. Apolipoprotein E/APOE 蛋白, Mouse (HEK293, His)

Apolipoprotein E/APOE 蛋白, Mouse (HEK293, His)

目录号: HY-P7532
COA 产品使用指南 技术支持

Apolipoprotein E/APOE 是连接脂质代谢和神经功能的关键分子。Apolipoprotein E/APOE 基因多态性通过影响蛋白结构和功能,与阿尔茨海默病、动脉粥样硬化等疾病密切相关。Apolipoprotein E/APOE 蛋白, Mouse (HEK293, His) 是重组的 Apolipoprotein E/APOE 蛋白,由 HEK293 表达,带 C-6*His 标签。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
Free Sample   Apply now  
5 μg ¥670 In-stock
10 μg ¥1150 In-stock
50 μg ¥3200 In-stock
100 μg ¥5440 In-stock
250 μg ¥8000 In-stock
500 μg ¥10500 In-stock
> 500 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

Apolipoprotein E/APOE is a key molecule linking lipid metabolism and neurological function. Apolipoprotein E/APOE gene polymorphisms are closely associated with diseases such as Alzheimer’s disease and atherosclerosis by influencing protein structure and function. Apolipoprotein E/APOE Protein, Mouse (HEK293, His) is a recombinant APOE protein expressed in HEK293 cells, with a C-6*His tag[1][2][3].

研究背景

Apolipoprotein E/APOE 是可溶性载脂蛋白家族成员,主要在肝脏和脑组织中合成。肝源性 Apolipoprotein E/APOE 参与外周脂质代谢,脑内 Apolipoprotein E/APOE 由星形胶质细胞产生,参与神经元修复与突触可塑性。Apolipoprotein E/APOE 通过与低密度脂蛋白受体家族相互作用,在血浆和中枢神经系统脂质运输中起关键作用。Apolipoprotein E/APOE 三种常见异构体 (APOE2、APOE3、APOE4) 因 112 和 158 位氨基酸差异,对动脉粥样硬化和阿尔茨海默病等疾病风险影响不同。APOE4 因结构域相互作用和稳定性较低是阿尔茨海默病的主要遗传风险因素,而 APOE2 因受体结合弱与 Ⅲ 型高脂蛋白血症相关[1][2][3]

生物活性

Measured in a cell proliferation assay using SH-SY5Y Human neuroblastoma cells. The ED50 this effect is 15-50 ng/mL.

  • Measured in a cell proliferation assay using SH-SY5Y Human neuroblastoma cells. The ED50 for this effect is 41.72 ng/ml, corresponding to a specific activity is 2.39×104 units/mg.
种属

Mouse

表达系统

HEK293

标签

C-6*His

蛋白编号

P08226 (E19-Q311)

基因 ID
蛋白结构
N-term
APOE (E19-Q311)
Accession # P08226
6*His
C-term
蛋白长度

Full Length of Mature Protein

中文名
Apolipo 蛋白 E 蛋白/APOE 蛋白
同用名
rMuApolipoprotein E, His; ApoE; Apolipoprotein E
氨基酸序列

EGEPEVTDQLEWQSNQPWEQALNRFWDYLRWVQTLSDQVQEELQSSQVTQELTALMEDTMTEVKAYKKELEEQLGPVAEETRARLGKEVQAAQARLGADMEDLRNRLGQYRNEVHTMLGQSTEEIRARLSTHLRKMRKRLMRDAEDLQKRLAVYKAGAREGAERGVSAIRERLGPLVEQGRQRTANLGAGAAQPLRDRAQAFGDRIRGRLEEVGNQARDRLEEVREHMEEVRSKMEEQTQQIRLQAEIFQARLKGWFEPIVEDMHRQWANLMEKIQASVATNPIITPVAQENQHHHHHH

分子量

Approximately 34-40 kDa

纯度
  • Greater than 90% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4 or PBS, pH 7.4, 8% trehalose.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

Apolipoprotein E/APOE Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
Apolipoprotein E/APOE Protein, Mouse (HEK293, His)
目录号:
HY-P7532
需求量: