ATG14 蛋白, Human (Myc, His)

ATG14 is critical for basal and induced autophagy, determining PI3KC3-C1 localization, helping autophagosome formation and BECN1 translocation. It enhances PIK3C3 activity in a BECN1-dependent manner, which is essential for BECN1 phosphorylation. ATG14 Protein, Human (Myc, His) is the recombinant human-derived ATG14 protein, expressed by E. coli , with C-Myc, N-10*His labeled tag. The total length of ATG14 Protein, Human (Myc, His) is 492 a.a., with molecular weight of ~70 kDa.

ATG14 对于基础自噬和诱导自噬都是必需的，在确定自噬特异性 PI3 激酶复合物 PI3KC3-C1 的定位方面发挥着关键作用。该蛋白有助于自噬体形成以及 MAP1LC3/LC3 与磷脂酰乙醇胺的结合，同时促进 BECN1 从反高尔基体网络易位至自噬体。ATG14 以 BECN1 依赖性方式增强 PIK3C3 活性，并且对于 BECN1 的自噬依赖性磷酸化至关重要。它刺激 BECN1 磷酸化，但通过 AMPK 抑制 PIK3C3 磷酸化。此外，ATG14 与自噬体上的 STX17-SNAP29 二元 t-SNARE 复合物结合，引发 VAMP8 相互作用，促进自噬体-内溶酶体融合。ATG14 的同源寡聚化对于其在膜束缚和增强 SNARE 介导的融合中的作用至关重要。作为 PI3K 复合物 I 的组成部分，ATG14 与催化亚基 PIK3C3、调节亚基 PIK3R4 和 BECN1 相关，呈现 V 形结构。ATG14 与各种伙伴相互作用，包括 PIK3CB、BECN2、STX17-SNAP29 二元 t-SNARE 复合物和 NRBF2，强调其在自噬过程和细胞功能中的多方面参与。TMEM39A 缺失会影响与 PIK3C3 和 BECN1 的相互作用，这强调了自噬中 ATG14 的复杂调控网络。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

C-Myc;N-10*His

Q6ZNE5 (M1-R492)

22863  [NCBI]

MASPSGKGARALEAPGCGPRPLARDLVDSVDDAEGLYVAVERCPLCNTTRRRLTCAKCVQSGDFVYFDGRDRERFIDKKERLSRLKSKQEEFQKEVLKAMEGKWITDQLRWKIMSCKMRIEQLKQTICKGNEEMEKNSEGLLKTKEKNQKLYSRAQRHQEKKEKIQRHNRKLGDLVEKKTIDLRSHYERLANLRRSHILELTSVIFPIEEVKTGVRDPADVSSESDSAMTSSTVSKLAEARRTTYLSGRWVCDDHNGDTSISITGPWISLPNNGDYSAYYSWVEEKKTTQGPDMEQSNPAYTISAALCYATQLVNILSHILDVNLPKKLCNSEFCGENLSKQKFTRAVKKLNANILYLCFSQHVNLDQLQPLHTLRNLMYLVSPSSEHLGRSGPFEVRADLEESMEFVDPGVAGESDESGDERVSDEETDLGTDWENLPSPRFCDIPSQSVEVSQSQSTQASPPIASSSAGGMISSAAASVTSWFKAYTGHR

Approximately 70 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.