BCL2 蛋白, Human (His)

BCL2 Protein regulates the mitochondrial membrane permeability, and inhibits the caspase activity, thereby suppressing the apoptosis in various cell types. BCL2 Protein interacts with BECN1 and AMBRA1, and inhibits the autophagy. BCL2 Protein, Human (His) is the human-derived resombinant BCL2 Protein that is expressed in in E. coli with a His tag at the C-terminus.

关键调节因子包括 Bcl-2 家族的蛋白质，其中一些（例如 Bcl-2、Bcl-xL、Mcl-1 和 A1）促进细胞存活，而另一些（例如 Bax 和 Bak）则拮抗细胞存活。细胞凋亡调节紊乱与癌症、自身免疫性疾病和退行性疾病密切相关^[1]。

BCL2 Protein, Human（1 mg/小鼠，腹腔注射，每 12 小时一次，持续3天）可减少心脏和肠道中的细胞凋亡数量，在盲肠结扎穿刺 (CLP) 诱发的脓毒症模型中提高小鼠的存活率^[2]。
BCL2 Protein, Human（1 mg/小鼠，腹腔注射，单剂量）可减少缺血-再灌注 (I/R) 损伤后的细胞凋亡，在小鼠缺血-再灌注模型中减轻后肢骨骼肌和心肌的 I/R 损伤^[3]。

Measured by its binding ability in a functional ELISA. When Recombinant Human BCL2 is present at 10 μg/mL, can bind Recombinant Mouse Bcl-XL. The ED₅₀ for this effect is 0.1426-0.1441 μg/mL.

• 
  Measured by its binding ability in a functional ELISA. When Recombinant Human BCL2 is present at 10 μg/mL, can bind Recombinant Mouse Bcl-XL. The ED₅₀ for this effect is 0.1441 μg/mL.

Human

E. coli

C-6*His

P10415-1 (M1-D211)

596  [NCBI]

Partial

MAHAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDVGAAPPGAAPAPGIFSSQPGHTPHPAASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHLTLRQAGDDFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVNREMSPLVDNIALWMTEYLNRHLHTWIQDNGGWDAFVELYGPSMRPLFD

Approximately 22-27 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE

溶液

Supplied as a 0.2 μm filter solution of 50 mM Tris-HCl, 10% glycerin, pH 8.0 or 50 mM Tris-HCL, 150 mM NaCl, 100mM arginine, pH 8.0 , 20% Glycerol or PBS, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Print CG, et al. Apoptosis regulator bcl-w is essential for spermatogenesis but appears otherwise redundant. Proc Natl Acad Sci U S A. 1998 Oct 13;95(21):12424-31.  [Content Brief]

  [2]. Iwata A, et al., Extracellular administration of BCL2 protein reduces apoptosis and improves survival in a murine model of sepsis. PLoS One. 2011 Feb 24;6(2):e14729.  [Content Brief]

  [3]. Iwata A, et al., Extracellular BCL2 proteins are danger-associated molecular patterns that reduce tissue damage in murine models of ischemia-reperfusion injury. PLoS One. 2010 Feb 8;5(2):e9103.  [Content Brief]