1. 重组蛋白
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  3. BRD3 蛋白, Human (His)

BRD3 蛋白, Human (His) 是重组的 BRD3 蛋白,由 E. coli 表达,带有 His 标签。

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描述

BRD3 Protein, Human (His) is the recombinant human-derived BRD3, expressed by E. coli , with His labeled tag.

研究背景

BRD3 是一种染色质阅读器 (chromatin reader),能够识别并结合乙酰化组蛋白 (acetylated histones),从而调控基因表达并重塑染色质结构。它通过招募转录因子 (transcription factors) 和辅激活因子 (coactivators) 到靶基因位点,并激活 RNA 聚合酶 II (RNA polymerase II) 机制以促进转录延伸。体外实验表明,BRD3 能够结合组蛋白 H2A、H2B、H3 和 H4 N 末端的乙酰化赖氨酸残基 (acetylated lysine residues)。在内胚层分化 (endoderm differentiation) 过程中,BRD3 通过与长链非编码 RNA (lncRNA) DIGIT 结合,发生液-液相分离 (liquid-liquid phase separation),进而促进其与组蛋白 H3 第 18 位赖氨酸乙酰化 (H3K18ac) 的结合,诱导内胚层基因表达。此外,BRD3 还能结合非组蛋白乙酰化蛋白 (non-histones acetylated proteins),例如 GATA1 和 GATA2 (by similar):通过促进转录因子 GATA1 与其靶标的结合来调控转录。

种属

Human

表达系统

E. coli

标签

His

蛋白编号

Q15059-1 (P29-V145)

基因 ID

8019

蛋白长度

Partial

中文名
BRD3 蛋白
同用名
KIAA0043; RING3L
预测分子量
16.6 kDa
纯度

Greater than 85% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

BRD3 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
BRD3 Protein, Human (His)
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HY-P703596
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