1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. BSSP-4 蛋白, Human (HEK293, His)

BSSP-4 蛋白, Human (HEK293, His)

目录号: HY-P70108
产品使用指南

BSSP-4 蛋白因其在裂解合成底物 HD-Leu-Thr-Arg-pNA 方面比甲苯磺酰基-Gly-Pro-Arg-pNA 具有特殊偏好而闻名。这种酶选择性表明 BSSP-4 对特定肽序列的潜在调节作用,强调了其在生物过程中的相关性。BSSP-4 蛋白, Human (HEK293, His) 是重组的 BSSP-4 蛋白,由 HEK293 表达,带有 C-6*His 标签。BSSP-4 蛋白, Human (HEK293, His) 全长 285 个氨基酸,分子量为 33-35 kDa。

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描述

BSSP-4 Protein is notable for its specific preference in cleaving the synthetic substrate H-D-Leu-Thr-Arg-pNA over tosyl-Gly-Pro-Arg-pNA. This enzymatic selectivity indicates BSSP-4's potential modulation of specific peptide sequences, emphasizing its relevance in biological processes. BSSP-4 Protein, Human (HEK293, His) is the recombinant human-derived BSSP-4 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of BSSP-4 Protein, Human (HEK293, His) is 285 a.a., with molecular weight of 33-35 kDa.

研究背景

BSSP-4 蛋白占据了中心舞台,因为与甲苯磺酰基-甘氨酸-脯氨酸-精氨酸-pNA 相比,它对切割合成底物 HD-Leu-Thr-Arg-pNA 表现出明显的偏好。这种酶特异性突出了 BSSP-4 在底物识别和切割方面的选择性,展示了其在调节特定肽序列中的潜在作用。HD-Leu-Thr-Arg-pNA 优于甲苯磺酰基-Gly-Pro-Arg-pNA 表明底物特异性可能与蛋白质在生物过程中的功能作用相关。

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

Q9GZN4 (A33-S317)

基因 ID
蛋白结构
N-term
BSSP-4 (A33-S317)
Accession # Q9GZN4
6*His
C-term
同用名
rHuBrain-specific serine protease 4/BSSP-4, His; Brain-Specific Serine Protease 4; BSSP-4; Serine Protease 22; Serine Protease 26; Tryptase Epsilon; PRSS22; BSSP4; PRSS26
氨基酸序列

ARIPVPPACGKPQQLNRVVGGEDSTDSEWPWIVSIQKNGTHHCAGSLLTSRWVITAAHCFKDNLNKPYLFSVLLGAWQLGNPGSRSQKVGVAWVEPHPVYSWKEGACADIALVRLERSIQFSERVLPICLPDASIHLPPNTHCWISGWGSIQDGVPLPHPQTLQKLKVPIIDSEVCSHLYWRGAGQGPITEDMLCAGYLEGERDACLGDSGGPLMCQVDGAWLLAGIISWGEGCAERNRPGVYISLSAHRSWVEKIVQGVQLRGRAQGGGALRAPSQGSGAAARS

分子量

33-35 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

BSSP-4 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
BSSP-4 Protein, Human (HEK293, His)
目录号:
HY-P70108
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