Caspase-3/CASP3 蛋白, Human (His)

Caspase-3/CASP3 protein is critical in the execution of apoptosis, cleaving PARP1 and activating SREBP, caspase-6, -7 and -9, initiating the cascade leading to apoptosis. In addition to apoptosis, it regulates cell adhesion, promotes inflammation, inhibits AKT1 in oxidative stress, and modulates antiviral responses by cleaving proteins such as CGAS, IRF3, and MAVS. Caspase-3/CASP3 Protein, Human (His) is the recombinant human-derived Caspase-3/CASP3 protein, expressed by E. coli , with C-His labeled tag.

Caspase-3 是细胞凋亡执行途径的关键参与者，在多种细胞过程中发挥着核心作用。在细胞凋亡启动过程中，它协调聚（ADP-核糖）聚合酶 PARP1 的蛋白水解裂解，例证了其对关键细胞成分的调节影响。这种多功能蛋白质通过裂解和激活甾醇调节元件结合蛋白 (SREBP)、caspase-6、-7 和 -9 来扩展其作用范围，从而参与导致细胞凋亡的级联事件。除了其经典的细胞凋亡功能之外，Caspase-3 还通过 RET 裂解参与交感神经元内的细胞粘附，并通过裂解 IL-1 beta 促进炎症环境。为了应对氧化应激，它会抑制丝氨酸/苏氨酸蛋白激酶 AKT1。此外，Caspase-3 通过裂解 CGAS、IRF3 和 MAVS 等蛋白质，在调节抗病毒反应中发挥作用，抑制 I 型干扰素的产生。此外，它通过裂解和激活gasdermin-E (GSDME) 以及通过XRCC4 和磷脂扰乱酶蛋白裂解调节凋亡细胞表面的磷脂酰丝氨酸暴露，在细胞焦亡中发挥作用。这种全面的作用强调了 Caspase-3 在细胞稳态和对不同刺激的反应中复杂的调节功能。

Measured by its ability to cleave the fluorogenic peptide substrate Ac-DEVD-AFC (HY-P1005) and the specific activity is > 5000 pmoles/min/μg. (The proenzyme needs to be activated by Caspase-8 for an activated form.)

Human

E. coli

C-6*His

P42574 (S29-H277)

836

Full Length of Mature Protein

SGISLDNSYKMDYPEMGLCIIINNKNFHKSTGMTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELMRDVSKEDHSKRSSFVCVLLSHGEEGIIFGTNGPVDLKKITNFFRGDRCRSLTGKPKLFIIQACRGTELDCGIETDSGVDDDMACHKIPVEADFLYAYSTAPGYYSWRNSKDGSWFIQSLCAMLKQYADKLEFMHILTRVNRKVATEFESFSFDATFHAKKQIPCIVSMLTKELYFYH

Approximately 29 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from sterile PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping with dry ice.