CD64 蛋白, Human (Biotinylated, 272a.a, HEK293, His-Avi)

CD64 protein, a high affinity receptor, mediates IgG effector functions, triggering antibody-dependent cellular cytotoxicity (ADCC). It interacts with IGHG1 and forms a signaling complex with FCERG1, contributing to immune responses. CD64 also interacts with FLNA, EPB41L2, LAT, PPL, HCK, and LYN to prevent degradation of FCGR1A and regulate immune function. CD64 Protein, Human (Biotinylated, 272a.a, HEK293, His-Avi) is the recombinant human-derived CD64 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of CD64 Protein, Human (Biotinylated, 272a.a, HEK293, His-Avi) is 272 a.a., with molecular weight of 34-68 kDa.

CD64 蛋白是免疫球蛋白 γ Fc 区的高亲和力受体。它在先天性和适应性免疫反应中发挥作用。CD64 介导单核细胞上的 IgG 效应器功能，触发针对病毒感染细胞的抗体依赖性细胞毒性 (ADCC)。它与 IGHG1 相互作用并与 FCERG1 形成功能信号复合物。CD64 还与 FLNA 相互作用，防止 FCGR1A 降解。此外，它还与 EPB41L2、LAT、PPL、HCK 和 LYN 相互作用，从而发挥其在免疫调节中的多种功能。

1.Immobilized Anti-CD20(Ro) Antibody (Rituximab) at 1 μg/mL (100 μL/well) can bind Recombinant Human CD64 / FCGR1A Protein (His & AVI Tag), Biotinylated, the EC₅₀ is 1-3 ng/mL.
2.Biotinylated Human Fc gamma RI, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Trastuzumab with an affinity constant of 5 nM as determined in SPR assay (Biacore T200).

Human

HEK293

C-Avi;C-His

P12314 (Q16-T287)

2209  [NCBI]

MWFLTTLLLWVPVDGQVDTTKAviTLQPPWVSVFQEETVTLHCEVLHLPGSSSTQWFLNGTATQTSTPSYRITSASVNDSGEYRCQRGLSGRSDPIQLEIHRGWLLLQVSSRVFTEGEPLALRCHAWKDKLVYNVLYYRNGKAFKFFHWNSNLTILKTNISHNGTYHCSGMGKHRYTSAGISVTVKELFPAPVLNASVTSPLLEGNLVTLSCETKLLLQRPGLQLYFSFYMGSKTLRGRNTSSEYQILTARREDSGLYWCEAATEDGNVLKRSPELELQVLGLQLPT

34-68 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.