EBP1 蛋白, Human (His)

EBP1 protein participates in the signal transduction pathway regulated by ERBB3 and is related to growth regulation. As an androgen receptor (AR) corepressor, it is regulated by the ERBB3 ligand neregulin-1/heregulin (HRG). EBP1 Protein, Human (His) is the recombinant human-derived EBP1 protein, expressed by E. coli , with N-His labeled tag. The total length of EBP1 Protein, Human (His) is 393 a.a., with molecular weight of ~32 kDa.

EBP1 蛋白在 ERBB3 调节的信号转导途径中发挥作用，并与生长调节相关。它作为雄激素受体 (AR) 的辅阻遏物，并受 ERBB3 配体 neregulin-1/heregulin (HRG) 调节。此外，EBP1 可能通过招募组蛋白乙酰化酶 (HAT) 活性来抑制一些 E2F1 调节的启动子的转录。它表现出 RNA 结合能力，并与各种成熟和前体 rRNA 相关，表明参与 rRNA 加工和核糖体组装的中间和后期步骤的调节。此外，EBP1 介导特定病毒内部核糖体进入位点 (IRES) 的帽独立翻译。从功能上讲，它在调节细胞增殖、分化和存活中发挥作用，对细胞凋亡抑制（异构体 1）和促进细胞分化（异构体 2）具有异构体特异性作用。该蛋白与各种伙伴相互作用，包括 ERBB3 的细胞质结构域，并表现出与 AR、NCL/核仁素、HDAC2、RB1、AKT1、RNF20、HUWE1 和 DNAJC21 的动态关联，突出了其与多种细胞成分结合和参与参与的多功能性。复杂的分子过程。

Human

E. coli

N-His

Q9UQ80-1 (S2-D394)

5036  [NCBI]

SGEDEQQEQTIAEDLVVTKYKMGGDIANRVLRSLVEASSSGVSVLSLCEKGDAMIMEETGKIFKKEKEMKKGIAFPTSISVNNCVCHFSPLKSDQDYILKEGDLVKIDLGVHVDGFIANVAHTFVVDVAQGTQVTGRKADVIKAAHLCAEAALRLVKPGNQNTQVTEAWNKVAHSFNCTPIEGMLSHQLKQHVIDGEKTIIQNPTDQQKKDHEKAEFEVHEVYAVDVLVSSGEGKAKDAGQRTTIYKRDPSKQYGLKMKTSRAFFSEVERRFDAMPFTLRAFEDEKKARMGVVECAKHELLQPFNVLYEKEGEFVAQFKFTVLLMPNGPMRITSGPFEPDLYKSEMEVQDAELKALLQSSASRKTQKKKKKKASKTAENATSGETLEENEAGD

Approximately 45 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.