EIF4EBP2 蛋白, Human (His)

EIF4EBP2 is a translation initiation repressor protein that plays a critical regulatory role in synaptic plasticity, learning, and memory. In the hypophosphorylated state, EIF4EBP2 competes with EIF4G1/EIF4G3, forms a complex with EIF4E, and inhibits translation. EIF4EBP2 Protein, Human (His) is the recombinant human-derived EIF4EBP2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of EIF4EBP2 Protein, Human (His) is 120 a.a., with molecular weight of ~17.0 kDa.

EIF4EBP2 是翻译起始的抑制因子，在突触可塑性、学习和记忆形成中发挥着关键作用。作为 EIF4E 活性的关键调节因子，低磷酸化形式的 EIF4EBP2 与 EIF4G1/EIF4G3 竞争，与 EIF4E 形成强大的复合物，从而抑制翻译。相反，过度磷酸化形式与 EIF4E 分离，使 EIF4G1/EIF4G3 和 EIF4E 之间相互作用，最终启动翻译。EIF4EBP2 富含于大脑中，是突触活动和神经元干细胞更新的关键调节剂，有助于调节蛋白质翻译以响应各种信号通路，包括 MAP 激酶和 mTORC1。磷酸化事件和蛋白质相互作用之间错综复杂的相互作用强调了 EIF4EBP2 在协调细胞对外部刺激的反应中的多方面作用。

Human

E. coli

N-6*His

Q13542 (M1-I120)

1979  [NCBI]

MSSSAGSGHQPSQSRAIPTRTVAISDAAQLPHDYCTTPGGTLFSTTPGGTRIIYDRKFLLDRRNSPMAQTPPCHLPNIPGVTSPGTLIEDSKVEVNNLNNLNNHDRKHAVGDDAQFEMDI

Approximately 17.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0 .

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.