FAM20C 蛋白, Human (HEK293, Myc, His)

The FAM20C protein is a Golgi kinase that phosphorylates secretory pathway proteins and contributes to the biomineralization of bones and teeth. FAM20C phosphorylates caseins and proteins involved in biomineralization and also affects lipid homeostasis, wound healing, and cell migration and adhesion. FAM20C Protein, Human (HEK293, Myc, His) is the recombinant human-derived FAM20C protein, expressed by HEK293 , with N-His, C-Myc labeled tag. The total length of FAM20C Protein, Human (HEK293, Myc, His) is 492 a.a., with molecular weight of ~61.4 kDa.

FAM20C 蛋白作为高尔基体丝氨酸/苏氨酸蛋白激酶，在 Ser-x-Glu/pSer 基序内的分泌途径蛋白磷酸化中发挥关键作用，这对于骨骼和牙齿的生物矿化至关重要。作为细胞外蛋白的主要蛋白激酶，FAM20C 对细胞外磷酸化蛋白质组的生成有显着贡献，表现出对 Ser-x-Glu/pSer 基序内的蛋白质的偏好，同时还表现出更广泛的底物特异性。值得注意的是，FAM20C 磷酸化 ERO1A 以增强其活性，这对于维持内质网氧化还原稳态和促进氧化蛋白折叠至关重要。在内质网应激时，FAM20C 磷酸化 P4HB/PDIA1，诱导功能转换，将 P4HB 从氧化还原酶转变为分子伴侣，这对于维持 ER 蛋白质稳态和减少 ER 应激下的细胞死亡至关重要。此外，FAM20C 对于成骨细胞分化和矿化、磷酸化参与生物矿化的各种蛋白质（如 AMELX、AMTN、ENAM 和 SPP1/OPN）至关重要。除了在生物矿化中的作用外，FAM20C 还有助于脂质稳态、伤口愈合以及细胞迁移和粘附。

Human

HEK293

N-His;C-Myc

Q8IXL6 (93D-584R)

56975  [NCBI]

DFSSDPSSNLSSHSLEKLPPAAEPAERALRGRDPGALRPHDPAHRPLLRDPGPRRSESPPGPGGDASLLARLFEHPLYRVAVPPLTEEDVLFNVNSDTRLSPKAAENPDWPHAGAEGAEFLSPGEAAVDSYPNWLKFHIGINRYELYSRHNPAIEALLHDLSSQRITSVAMKSGGTQLKLIMTFQNYGQALFKPMKQTREQETPPDFFYFSDYERHNAEIAAFHLDRILDFRRVPPVAGRMVNMTKEIRDVTRDKKLWRTFFISPANNICFYGECSYYCSTEHALCGKPDQIEGSLAAFLPDLSLAKRKTWRNPWRRSYHKRKKAEWEVDPDYCEEVKQTPPYDSSHRILDVMDMTIFDFLMGNMDRHHYETFEKFGNETFIIHLDNGRGFGKYSHDELSILVPLQQCCRIRKSTYLRLQLLAKEEYKLSLLMAESLRGDQVAPVLYQPHLEALDRRLRVVLKAVRDCVERNGLHSVVDDDLDTEHRAASAR

Approximately 61.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.