G6B 蛋白, Human (HEK293, His)

The G6B protein is an inhibitory receptor critical for hematopoietic lineage differentiation, megakaryocyte function, and platelet production. Its effects include inhibiting platelet aggregation and activation induced by agonists such as ADP and collagen-related peptides. G6B Protein, Human (HEK293, His) is the recombinant human-derived G6B protein, expressed by HEK293 , with C-His labeled tag. The total length of G6B Protein, Human (HEK293, His) is 125 a.a., with molecular weight of ~18-23 kDa.

G6B 是一种抑制性受体，在调节造血谱系分化、巨核细胞功能和血小板生成中发挥着至关重要的作用。这种调节功能延伸至抑制由 ADP 和胶原相关肽等各种激动剂诱导的血小板聚集和激活。这种抑制是通过受体对 CLEC1B 和 GP6:FcRgamma 信号传导的影响来介导的，涉及两个基于免疫受体酪氨酸的抑制剂基序 (ITIM)。值得注意的是，G6B 以不依赖钙的方式发挥作用。亚型 B 包含跨膜区域和上述 ITIM，作为抑制性对应物，而亚型 A 被认为是亚型 B 的激活对应物。这种双亚型系统反映了造血和血小板功能背后的微妙调节机制。

Measured by its ability to induce cell death using Mv1Lu mink lung epithelial cells. The ED₅₀ for this effect is 2.344 μg/mL, corresponding to a specific activity is 4.266×10^2 U/mg.

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  Measured by its ability to induce cell death using Mv1Lu mink lung epithelial cells. The ED₅₀ for this effect is 2.344 μg/mL, corresponding to a specific activity is 4.266×10² U/mg.

Human

HEK293

C-6*His

O95866/NP_612116.1 (N18-Q142)

80739  [NCBI]

NPGASLDGRPGDRVNLSCGGVSHPIRWVWAPSFPACKGLSKGRRPILWASSSGTPTVPPLQPFVGRLRSLDSGIRRLELLLSAGDSGTFFCKGRHEDESRTVLHVLGDRTYCKAPGPTHGSVYPQ

Approximately 18-23 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.