GGT5 蛋白, Human (HEK293, His)

The GGT5 protein is an important glycosaminoglycan lyase that selectively cleaves heparin and heparan sulfate using a β-elimination mechanism. It targets specific linkages, such as alpha-D-GlcNp2S6S(1->4) alpha-L-IdoAp2S in heparin and alpha-D-GlcNp2Ac(or 2S)6OH(1->4)beta- in heparan sulfate D-GlcAp. GGT5 Protein, Human (HEK293, His) is the recombinant human-derived GGT5 protein, expressed by HEK293 , with N-His labeled tag. The total length of GGT5 Protein, Human (HEK293, His) is 557 a.a., with molecular weight of ~23-27 & 48-61.8 KDa.

肝素酶 II 是一种糖胺聚糖裂解蛋白，利用 β-消除机制裂解肝素和硫酸乙酰肝素。具体来说，它针对 α-D-GlcNp2S6S(1->4) α-L-IdoAp2S 连接处的肝素和 α-D-GlcNp2Ac(or 2S)6OH(1->4)beta-D-GlcAp 处的硫酸乙酰肝素连锁。这种酶活性有助于调节糖胺聚糖的组成和功能，从而影响受这些分子影响的细胞过程。精确的切割位点强调了肝素酶 II 在修饰肝素和硫酸乙酰肝素结构方面的特异性，暗示其在涉及这些糖胺聚糖的不同生理和病理环境中的作用。

Measured by its ability to hydrolyze glutathione to Glu and Cys-Gly. The specific activity is 881.5814 pmol/min/µg, as measured under the described conditions.

Human

HEK293

N-10*His

P36269-1/NP_004112.2 (S30-Y586)

2687  [NCBI]

SRHQAPCGPQAFAHAAVAADSKVCSDIGRAILQQQGSPVDATIAALVCTSVVNPQSMGLGGGVIFTIYNVTTGKVEVINARETVPASHAPSLLDQCAQALPLGTGAQWIGVPGELRGYAEAHRRHGRLPWAQLFQPTIALLRGGHVVAPVLSRFLHNSILRPSLQASTLRQLFFNGTEPLRPQDPLPWPALATTLETVATEGVEVFYTGRLGQMLVEDIAKEGSQLTLQDLAKFQPEVVDALEVPLGDYTLYSPPPPAGGAILSFILNVLRGFNFSTESMARPEGRVNVYHHLVETLKFAKGQRWRLGDPRSHPKLQNASRDLLGETLAQLIRQQIDGRGDHQLSHYSLAEAWGHGTGTSHVSVLGEDGSAVAATSTINTPFGAMVYSPRTGIILNNELLDLCERCPRGSGTTPSPVSGDRVGGAPGRCWPPVPGERSPSSMVPSILINKAQGSKLVIGGAGGELIISAVAQAIMSKLWLGFDLRAAIAAPILHVNSKGCVEYEPNFSQEVQRGLQDRGQNQTQRPFFLNVVQAVSQEGACVYAVSDLRKSGEAAGY

Approximately 23-27 & 48-61.8 kDa due to the glycosylation

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.