ITIH3 蛋白, Human (HEK293, His)

The ITIH3 protein has a dual role: as a carrier of hyaluronic acid in serum and as a binding protein that facilitates interactions with various matrix proteins. These interactions regulate the localization, synthesis, and degradation of hyaluronic acid, which is critical for multiple cellular activities. ITIH3 Protein, Human (HEK293, His) is the recombinant human-derived ITIH3 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of ITIH3 Protein, Human (HEK293, His) is 617 a.a., with molecular weight of ~90.0 kDa.

ITIH3 蛋白表现出多种作用，可能充当血清中透明质酸的载体，并作为结合蛋白促进透明质酸与各种基质蛋白（包括组织中细胞表面存在的蛋白）之间的相互作用。这些相互作用在调节透明质酸的定位、合成和降解方面发挥着关键作用，这些过程对于细胞进行多种生物活动至关重要。在 I-α-I 血浆蛋白酶抑制剂的背景下，ITIH3 有助于这些抑制剂的组装，与一条或两条重链 (HC) 和轻链 Bikunin 形成复合物。值得注意的是，前α-抑制剂（P-α-I）由ITIH3/HC3和bikunin组成，强调了ITIH3在这些血浆蛋白酶抑制剂的结构组成中的整体参与。

Human

HEK293

C-6*His

Q06033 (L35-D651)

3699  [NCBI]

LPEGVANGIEVYSTKINSKVTSRFAHNVVTMRAVNRADTAKEVSFDVELPKTAFITNFTLTIDGVTYPGNVKEKEVAKKQYEKAVSQGKTAGLVKASGRKLEKFTVSVNVAAGSKVTFELTYEELLKRHKGKYEMYLKVQPKQLVKHFEIEVDIFEPQGISMLDAEASFITNDLLGSALTKSFSGKKGHVSFKPSLDQQRSCPTCTDSLLNGDFTITYDVNRESPGNVQIVNGYFVHFFAPQGLPVVPKNVAFVIDISGSMAGRKLEQTKEALLRILEDMQEEDYLNFILFSGDVSTWKEHLVQATPENLQEARTFVKSMEDKGMTNINDGLLRGISMLNKAREEHRIPERSTSIVIMLTDGDANVGESRPEKIQENVRNAIGGKFPLYNLGFGNNLNYNFLENMALENHGFARRIYEDSDADLQLQGFYEEVANPLLTGVEMEYPENAILDLTQNTYQHFYDGSEIVVAGRLVDEDMNSFKADVKGHGATNDLTFTEEVDMKEMEKALQERDYIFGNYIERLWAYLTIEQLLEKRKNAHGEEKENLTARALDLSLKYHFVTPLTSMVVTKPEDNEDERAIADKPGEDAEATPVSPAMSYLTSYQPPQNPYYYVDGD

Approximately 90.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.