1. 重组蛋白
  2. Others
  3. HMPV 蛋白, Human (HEK293, His)

HMPV 蛋白, Human (HEK293, His)

目录号: HY-P703870
产品使用指南 技术支持

融合糖蛋白 F0 被裂解生成成熟的 F1 和 F2 融合糖蛋白。它通过不同的构象状态转变以促进病毒和细胞膜的融合。HMPV 蛋白, Human (HEK293, His) 是重组的 HMPV 蛋白,由 HEK293 表达,带有 C-His 标签。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 μg ¥1280 1 - 2 周
50 μg ¥3600 1 - 2 周
100 μg ¥5800 1 - 2 周
> 100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Fusion glycoprotein F0 is cleaved to generate mature F1 and F2 fusion glycoproteins. It transitions through different conformational states to promote the fusion of virus and cell membranes. HMPV Protein, Human (HEK293, His) is the recombinant virus-derived HMPV protein, expressed by HEK293, with C-His labeled tag.

研究背景

融合糖蛋白 F0 是一种无活性前体,经过裂解生成成熟的 F1 和 F2 融合糖蛋白,代表 I 类病毒融合蛋白。根据目前的模型,该蛋白质至少表现出三种构象状态:融合前天然状态、发夹前中间状态和融合后发夹状态。在病毒和浆细胞膜融合事件中,卷曲螺旋区域采用发夹三聚体结构,将融合肽对齐到胞外域的 C 末端区域附近。这种结构的形成驱动病毒和细胞膜的并置和随后的融合,促进核衣壳递送到细胞质中。值得注意的是,这种融合过程与 pH 值无关,并且发生在质膜或内体膜上。F1-F2 三聚体(F 蛋白)还通过与宿主硫酸乙酰肝素结合,在宿主细胞附着中发挥关键作用,突出了其在介导病毒进入和感染中的多方面作用。

种属

Virus

表达系统

HEK293

标签

C-His

蛋白编号

Q6WB98 (L19-T489)

基因 ID

/

蛋白长度

Partial

中文名
HMPV 蛋白
同用名
Fusion glycoprotein F0; Protein F
预测分子量
57.2 kDa
分子量

Approximately 70.2 kDa & 50.5 kDa & 17.2 kDa,based on SDS-PAGE under reducing conditions.

糖基化修饰
Yes
纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

HMPV Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
HMPV Protein, Human (HEK293, His)
目录号:
HY-P703870
需求量: