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  3. NTN1 蛋白, Human (HEK293, His)

NTN1 蛋白, Human (HEK293, His)

目录号: HY-P704176
产品使用指南 技术支持

Netrin-1 蛋白是一种关键的轴突引导调节剂,可引导中枢神经系统的连合轴突和外周运动轴突。与 DCC 或 UNC5 受体的结合决定轴突的吸引或排斥。NTN1 蛋白, Human (HEK293, His) 是重组的 NTN1 蛋白,由 HEK293 表达,带有 C-His 标签。

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规格 价格 是否有货 数量
100 μg ¥8400 1 - 2 周
> 100 μg   询价  

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描述

Netrin-1 protein is a key axon guidance regulator that guides commissural and peripheral motor axons in the central nervous system. Binding to DCC or UNC5 receptors determines axonal attraction or repulsion. NTN1 Protein, Human (HEK293, His) is the recombinant human-derived NTN1 protein, expressed by HEK293, with C-His labeled tag.

研究背景

Netrin-1 是中枢神经系统 (CNS) 和外周运动神经元轴突引导的重要调节因子,在引导 CNS 内的连合轴突和外周运动神经元的运动轴突方面发挥着关键作用。Netrin-1 与 DCC 或某些 UNC5 受体的关联决定了轴突是被吸引还是被排斥。具体来说,与 UNC5C 的结合诱导 UNC5C 从微管中聚合的 TUBB3 解离,导致微管动力学增强和随后的轴突排斥。Netrin-1 对于背根神经节轴突向脊髓投射至关重要,并通过与受体相互作用来防止细胞凋亡的启动,从而发挥生存因子的作用。此外,Netrin-1 参与肿瘤发生强调了其在细胞凋亡中的调节作用。该蛋白与一系列受体结合,包括 DCC、UNC5A、UNC5B、UNC5C、UNC5D 和 DSCAM,并表现出与 APP 的相互作用,突出了其在细胞过程中的多方面作用。

种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

O95631-1 (V22-A604)

基因 ID

9423

蛋白结构
N-term
NTN1 (V22-A604)
Accession # O95631-1
His
C-term
蛋白长度

Full Length of Isoform-1 Mature Protein

中文名
人神经导向蛋白-1
同用名
Netrin-1,NTN1,NTN1L,Netrin1,netrin 1
氨基酸序列

VRGGPGLSMFAGQAAQPDPCSDENGHPRRCIPDFVNAAFGKDVRVSSTCGRPPARYCVVSERGEERLRSCHLCNASDPKKAHPPAFLTDLNNPHNLTCWQSENYLQFPHNVTLTLSLGKKFEVTYVSLQFCSPRPESMAIYKSMDYGRTWVPFQFYSTQCRKMYNRPHRAPITKQNEQEAVCTDSHTDMRPLSGGLIAFSTLDGRPSAHDFDNSPVLQDWVTATDIRVAFSRLHTFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHAARCVRDRDDSLVCDCRHNTAGPECDRCKPFHYDRPWQRATAREANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGYYRDMGKPITHRKACKACDCHPVGAAGKTCNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPVAPPTTAASSVEEPEDCDSYCKASKGKLKINMKKYCKKDYAVQIHILKADKAGDWWKFTVNIISVYKQGTSRIRRGDQSLWIRSRDIACKCPKIKPLKKYLLLGNAEDSPDQSGIVADKSSLVIQWRDTWARRLRKFQQREKKGKCKKA

预测分子量
67.5 kDa
分子量

Approximately 80-100 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

糖基化修饰
Yes
构象/形态
Monomer
纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.22 μm filtered solution of PBS, pH7.4 with NaCl with trehalose as protectant.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

NTN1 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
NTN1 Protein, Human (HEK293, His)
目录号:
HY-P704176
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