NTN1 蛋白, Human (HEK293, His)

Netrin-1 protein is a key axon guidance regulator that guides commissural and peripheral motor axons in the central nervous system. Binding to DCC or UNC5 receptors determines axonal attraction or repulsion. NTN1 Protein, Human (HEK293, His) is the recombinant human-derived NTN1 protein, expressed by HEK293, with C-His labeled tag.

Netrin-1 是中枢神经系统 (CNS) 和外周运动神经元轴突引导的重要调节因子，在引导 CNS 内的连合轴突和外周运动神经元的运动轴突方面发挥着关键作用。Netrin-1 与 DCC 或某些 UNC5 受体的关联决定了轴突是被吸引还是被排斥。具体来说，与 UNC5C 的结合诱导 UNC5C 从微管中聚合的 TUBB3 解离，导致微管动力学增强和随后的轴突排斥。Netrin-1 对于背根神经节轴突向脊髓投射至关重要，并通过与受体相互作用来防止细胞凋亡的启动，从而发挥生存因子的作用。此外，Netrin-1 参与肿瘤发生强调了其在细胞凋亡中的调节作用。该蛋白与一系列受体结合，包括 DCC、UNC5A、UNC5B、UNC5C、UNC5D 和 DSCAM，并表现出与 APP 的相互作用，突出了其在细胞过程中的多方面作用。

Human

HEK293

C-His

O95631-1 (V22-A604)

9423

Full Length of Isoform-1 Mature Protein

VRGGPGLSMFAGQAAQPDPCSDENGHPRRCIPDFVNAAFGKDVRVSSTCGRPPARYCVVSERGEERLRSCHLCNASDPKKAHPPAFLTDLNNPHNLTCWQSENYLQFPHNVTLTLSLGKKFEVTYVSLQFCSPRPESMAIYKSMDYGRTWVPFQFYSTQCRKMYNRPHRAPITKQNEQEAVCTDSHTDMRPLSGGLIAFSTLDGRPSAHDFDNSPVLQDWVTATDIRVAFSRLHTFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHAARCVRDRDDSLVCDCRHNTAGPECDRCKPFHYDRPWQRATAREANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGYYRDMGKPITHRKACKACDCHPVGAAGKTCNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPVAPPTTAASSVEEPEDCDSYCKASKGKLKINMKKYCKKDYAVQIHILKADKAGDWWKFTVNIISVYKQGTSRIRRGDQSLWIRSRDIACKCPKIKPLKKYLLLGNAEDSPDQSGIVADKSSLVIQWRDTWARRLRKFQQREKKGKCKKA

Approximately 80-100 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH7.4 with NaCl with trehalose as protectant.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.