2. 重组蛋白
  3. Enzymes & Regulators
  4. Protease Inhibitors
  5. Serpin (Protease Inhibitor)
  6. Alpha-1 Antitrypsin 1-3
  7. Serpin A1c 蛋白, Mouse (HEK293, His)

Serpin A1c 蛋白, Mouse (HEK293, His)

目录号: HY-P71301
产品使用指南

Serpin A1c 蛋白是细胞调节的熟练守护者，特别是丝氨酸蛋白酶（特别是胰蛋白酶和糜蛋白酶）的抑制剂。它在阻断酶活性方面的功效与它对弹性蛋白酶的相对无效性形成鲜明对比。Serpin A1c 蛋白, Mouse (HEK293, His) 是重组的 Serpin A1c 蛋白，由 HEK293 表达，带有 C-6*His 标签。Serpin A1c 蛋白, Mouse (HEK293, His) 全长 389 个氨基酸，分子量为 45-60 kDa。

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规格 是否有货
50 μg   询价  
100 μg   询价  

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描述

The Serpin A1c protein is a skilled guardian of cellular regulation, specifically acting as an inhibitor of serine proteases, specifically trypsin and chymotrypsin. Its efficacy in blocking enzymatic activity contrasts with its relative ineffectiveness against elastase. Serpin A1c Protein, Mouse (HEK293, His) is the recombinant mouse-derived Serpin A1c protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Serpin A1c Protein, Mouse (HEK293, His) is 389 a.a., with molecular weight of 45-60 kDa.
研究背景

Serpin A1c 是复杂细胞调节领域的熟练守护者，承担着专门针对丝氨酸蛋白酶的抑制剂的角色。它的警惕目光延伸到胰蛋白酶和胰凝乳蛋白酶，在阻止它们的酶活性方面表现出卓越的功效。然而，它的威力发生了微妙的转变，表现出对弹性蛋白酶的相对无效。Serpin A1c 对特定丝氨酸蛋白酶的战略性抑制强调了其作为敏锐调节剂的重要性，有助于细胞环境内酶活性的微妙平衡。
种属

Mouse
表达系统

HEK293
标签

C-6*His
蛋白编号

Q00896 (E25-K413)
基因 ID

20702  [NCBI]
蛋白结构
N-term
Serpin A1c (E25-K413)
Accession # Q00896
6*His
C-term
同用名
Alpha-1-antitrypsin 1-3; Alpha-1 protease inhibitor 3; Alpha-1 protease inhibitor 6; Alpha-1-antitrypsin 1-6; Serine protease inhibitor 1-3; Serine protease inhibitor 1-6; Serine protease inhibitor A1c; Serpin A1c; Serpina1c; Dom3; Dom6; Spi1-3; Spi1-6
氨基酸序列

EDVQETDTSQKDQSPASHEIATNLGDFAISLYRELVHQSNTSNIFFSPVSIATAFAMLSLGSKGDTHTQILEGLQFNLTQTSEADIHKSFQHLLQTLNRPDSELQLSTGNGLFVNNDLKLVEKFLEEAKNHYQAEVFSVNFAESEEAKKVINDFVEKGTQGKIVEAVKKLDQDTVFALANYILFKGKWKKPFDPENTEEAEFHVDESTTVKVPMMTLSGMLDVHHCSTLSSWVLLMDYAGNATAVFLLPDDGKMQHLEQTLSKELISKFLLNRRRRLAQIHFPRLSISGEYNLKTLMSPLGITRIFNNGADLSGITEENAPLKLSQAVHKAVLTIDETGTEAAAVTVLLAVPYSMPPILRFDHPFLFIIFEEHTQSPLFVGKVVDPTHK

分子量

45-60 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Serpin A1c Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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