Serpin B4 蛋白, Human (sf9, His)

Serpin B4 is a protease inhibitor that may modulate the host immune response against tumor cells, suggesting a crucial role in fine-tuning immune balance. Its ability to inhibit proteases emphasizes its importance in influencing key regulatory pathways, enhancing the immune system's effectiveness against cancer cells. Serpin B4 Protein, Human (sf9, His) is the recombinant human-derived Serpin B4 protein, expressed by Sf9 insect cells , with N-His labeled tag.

Serpin B4 蛋白通过充当蛋白酶抑制剂，成为宿主针对肿瘤细胞的免疫反应的潜在调节剂。它作为调节剂的作用表明它在微调免疫反应的复杂平衡方面具有至关重要的功能，特别是在对抗肿瘤细胞的情况下。该蛋白质抑制蛋白酶的能力表明其影响关键调节途径的能力，可能增强免疫系统识别和应对癌细胞的有效性。Serpin B4 作为蛋白酶抑制剂，强调了其在免疫系统和肿瘤细胞之间动态相互作用中的重要性，使其成为进一步探索癌症免疫治疗的有希望的候选者。

Measured by its ability to inhibit CMA1 cleavage of 100 µM fluorogenic peptide substrate Suc-AAPF-AMC (HY-137811) that incubate at room temperature in kinetic mode for 5 minutes. The IC₅₀ is 15.514 nM.

Human

Sf9 insect cells

N-His

P48594 (M1-P390)

6318  [NCBI]

Full Length

Approximately 45 kDa

Greater than 88% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 20% Glycerol, 1 mM EDTA, 1 mM DTT. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.