Serpin F1 蛋白, Rat (HEK293, His)

Serpin F1 protein is part of the secreted Frizzled-related protein (sFRP) family and regulates the Wnt signaling pathway. As a member, Serpin F1 may interact with Wnt ligands and Frizzled receptors to regulate key cellular processes. Serpin F1 Protein, Rat (HEK293, His) is the recombinant rat-derived Serpin F1 protein, expressed by HEK293 , with C-His labeled tag.

SFRP2 蛋白是分泌型卷曲相关蛋白 (sFRP) 家族的成员，表明其参与调节 Wnt 信号通路。作为该家族的一部分，SFRP2 可能与 Wnt 配体和卷曲受体相互作用，对关键的细胞过程发挥调节作用。特征注释传播所需的保守残基的缺失强调了 sFRP 家族中 SFRP2 的独特结构特征。尽管缺乏某些保守元件，SFRP2 的特定属性可能有助于其在调节 Wnt 信号传导中发挥独特的作用，从而可能影响增殖、分化或组织发育等细胞活动。

Measured by its ability to enhance the adhesion of Saos-2 human osteosarcoma cells to bovine Collagen I coated plate. The ED₅₀ this effect is 0.178 μg/mL, corresponding to a specific activity is 5.62×10³ units/mg.

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  Measured by its ability to enhance the adhesion of Saos‑2 human osteosarcoma cells to bovine Collagen I coated plate. The ED₅₀ this effect is 0.178 μg/mL, corresponding to a specific activity is 5.62×10³ units/mg.

Rat

HEK293

C-His

Q80ZA3/NP_808788.1 (Q20-T418)

287526  [NCBI]

Full Length of Mature Protein

QNVPDSSQDSPAPDSTGEPVVEEDDPFFKAPVNKLAAAVSNFGYDLYRLRSGAVSTGNILLSPLSVATALSALSLGAEQRTESVIHRALYYDLINNPDIHSTYKELLASVTAPEKNFKSASRIVFERKLRVKSSFVAPLEKSYGTRPRILTGNPRIDLQEINNWVQAQMKGKIARSTREMPSALSILLLGVAYFKGQWATKFDSRKTTLQDFHLDEDRTVRVPMMSDPKAILRYGLDSDLNCKIAQLPLTGSMSIIFFLPLTVTQNLTMIEESLTSEFVHDIDRELKTIQAVLTVPKLKLSYEGDVTNSLQDMKLQSLFESPDFSKITGKPVKLTQVEHRAAFEWNEEGAGTSSNPDLQPVRLTFPLDYHLNRPFIFVLRDTDTGALLFIGRILDPSST

Approximately 45.9 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.