1. 重组蛋白
  2. Enzymes & Regulators
  3. Protease Inhibitors
  4. Serpin (Protease Inhibitor)
  5. Alpha-1 Antitrypsin 1-4
  6. Serpina1d 蛋白, Mouse (HEK293)

Serpina1d 蛋白, Mouse (HEK293)

目录号: HY-P77484
COA 产品使用指南

Serpina1d 蛋白是细胞调节中的警惕哨兵,专门充当丝氨酸蛋白酶(特别是胰蛋白酶和胰凝乳蛋白酶)的抑制剂。它能有效地阻断它们的酶活性,但对弹性蛋白酶似乎相对无效。Serpina1d 蛋白, Mouse (HEK293) 是重组的 Serpina1d 蛋白,由 HEK293 表达,不带标签。Serpina1d 蛋白, Mouse (HEK293) 全长 413 个氨基酸,分子量约为 ~54 kDa。

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规格 价格 是否有货 数量
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5 μg ¥2000 In-stock
10 μg ¥3400 In-stock
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The Serpina1d protein is a vigilant sentinel in cellular regulation, acting specifically as an inhibitor of serine proteases, particularly trypsin and chymotrypsin. It effectively blocks their enzymatic activity but appears relatively ineffective against elastase. Serpina1d Protein, Mouse (HEK293) is the recombinant mouse-derived Serpina1d protein, expressed by HEK293 , with tag free. The total length of Serpina1d Protein, Mouse (HEK293) is 413 a.a., with molecular weight of ~54 kDa.

研究背景

Serpina1d 是细胞调控复杂领域中的一个警惕的哨兵,承担着专门针对丝氨酸蛋白酶的抑制剂的角色。它的警惕目光延伸到胰蛋白酶和胰凝乳蛋白酶,在阻止它们的酶活性方面表现出显着的功效。然而,它的威力发生了微妙的转变,表现出对弹性蛋白酶的相对无效。Serpina1d 对特定丝氨酸蛋白酶的战略性抑制强调了其作为敏锐调节剂的重要性,有助于细胞环境内酶活性的微妙平衡。

生物活性

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate Mca-Arg-Pro-Lys-Pro-Val-Glu-Nval-Trp-Arg-Lys (DNP)-NH2 and the IC50 value is < 15 nM.

种属

Mouse

表达系统

HEK293

标签

Tag Free

蛋白编号

Q00897 (M1-K413)

基因 ID

20703  [NCBI]

蛋白结构
N-term
Serpina1d (M1-K413)
Accession # Q00897
C-term
同用名
Alpha-1-antitrypsin 1-4; Serine protease inhibitor 1-4; Serpin A1d; Dom4; Spi1-4
分子量

Approximately 54 kDa

纯度

Greater than 85% as determined by reducing SDS-PAGE

性状

溶液.

组分

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Serpina1d Protein, Mouse (HEK293)
目录号:
HY-P77484
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