STUB1 蛋白, Human

STUB1 protein is an E3 ubiquitin protein ligase that cooperates with ATXN3 to regulate ubiquitin chain length on substrates and prevent chain extension. It ubiquitinates NOS1 through Hsp70/Hsp40 and regulates chaperone complexes (Hsp70, Hsc70, Hsp90). STUB1 Protein, Human is the recombinant human-derived STUB1 protein, expressed by E. coli , with tag free. The total length of STUB1 Protein, Human is 303 a.a., with molecular weight of ~33.0 kDa.

STUB1 蛋白是一种 E3 泛素蛋白连接酶，在靶向错误折叠的伴侣底物进行蛋白酶体降解方面发挥着关键作用，与 ATXN3 合作调节附着在 STUB1 底物上的泛素链长度并防止进一步的链延伸。它与 Hsp70 和 Hsp40 一起泛素化 NOS1，调节 Hsp70、Hsc70 和 Hsp90 等关键伴侣复合物的活性。此外，STUB1 介导非规范短泛素链向 HSPA8 的转移，而不影响其降解。该蛋白通过催化 DNA 聚合酶 beta (POLB) 的多泛素化、放大 HUWE1/ARF-BP1 依赖性单泛素化并导致 POLB 降解来参与碱基切除修复。STUB1 还参与 CYP3A4 的多泛素化，泛素化 EPHA2 以潜在地调节受体稳定性，充当 HSPA1A 和 HSPA1B 的共伴侣，并通过泛素介导的降解调节 SMAD3 水平来负调节 TGF-β 信号传导。此外，STUB1 有助于 FOXP3、SIRT6 和 RIPK3 的降解，并可能下调 PD-L1/CD274 质膜表达。其多样化的功能强调了其在细胞稳态和免疫调节中的重要性。

Human

E. coli

Tag Free

Q9UNE7 (M1-Y303)

10273  [NCBI]

MKGKEEKEGGARLGAGGGSPEKSPSAQELKEQGNRLFVGRKYPEAAACYGRAITRNPLVAVYYTNRALCYLKMQQHEQALADCRRALELDGQSVKAHFFLGQCQLEMESYDEAIANLQRAYSLAKEQRLNFGDDIPSALRIAKKKRWNSIEERRIHQESELHSYLSRLIAAERERELEECQRNHEGDEDDSHVRAQQACIEAKHDKYMADMDELFSQVDEKRKKRDIPDYLCGKISFELMREPCITPSGITYDRKDIEEHLQRVGHFDPVTRSPLTQEQLIPNLAMKEVIDAFISENGWVEDY

Approximately 33.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.