TIGAR 蛋白, Human (TAT)

TIGAR is a protein that helps cells survive stress by regulating glycolysis and promoting cell survival. It reduces reactive oxygen species (ROS) levels and protects against oxidative damage. TIGAR also plays a role in promoting tissue regeneration and has neuroprotective effects. In cancer cells, TIGAR promotes DNA repair and is involved in tumor progression. TIGAR Protein, Human (TAT) is the recombinant human-derived TIGAR protein, expressed by E. coli , with C-TAT labeled tag.

TIGAR（TP53 诱导的糖酵解和凋亡调节剂）作为果糖双磷酸酶发挥作用，水解果糖 2,6-二磷酸和果糖 1,6-二磷酸。作为糖酵解的负调节剂，TIGAR 以 p53/TP53 依赖性方式降低细胞内 2,6-二磷酸果糖水平，从而激活磷酸戊糖途径 (PPP) 并促进 NADPH 产生。这种调节活性通过降低活性氧 (ROS) 含量，有助于细胞防御氧化或代谢应激诱导的细胞死亡。TIGAR 的作用延伸到缺氧期间保护细胞，它通过涉及己糖激酶 2 (HK2) 的机制降低线粒体 ROS 水平，独立于果糖双磷酸酶活性。在心脏损伤应激等情况下，TIGAR 介导 p53 诱导的心肌细胞线粒体自噬抑制，影响 ROS 水平并加剧心肌细胞凋亡。此外，TIGAR 在成人肠道再生、针对缺血性脑损伤的神经保护以及通过响应缺氧和/或基因组应激诱导的 DNA 损伤而激活 PPP 来增强 DNA 修复来促进癌细胞存活方面发挥着重要作用。

Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP). The specific activity is 19.321 pmol/min/µg that incubate at 37°C for 3h.

Human

E. coli

C-TAT

Q9NQ88 (M1-R270)

57103  [NCBI]

Full length

MARFALTVVRHGETRFNKEKIIQGQGVDEPLSETGFKQAAAAGIFLNNVKFTHAFSSDLMRTKQTMHGILERSKFCKDMTVKYDSRLRERKYGVVEGKALSELRAMAKAAREECPVFTPPGGETLDQVKMRGIDFFEFLCQLILKEADQKEQFSQGSPSNCLETSLAEIFPLGKNHSSKVNSDSGIPGLAASVLVVSHGAYMRSLFDYFLTDLKCSLPATLSRSELMSVTPNTGMSLFIINFEEGREVKPTVQCICMNLQDHLNGLTETR

Approximately 32 kDa, based on SDS-PAGE under reducing conditions.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.22 μm filtered solution in 50 mM Tris-HCL, 300 mM NaCl, pH 8.0, 10% Glycerol.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.