TMED1 蛋白, Human (HEK293, His)

The TMED1 protein may facilitate vesicular protein trafficking in the early secretory pathway, acting as a cargo receptor on the luminal side to incorporate secretory cargo into transport vesicles. It is involved in the formation of the vesicle shell on the cytoplasmic side and positively affects IL-33-mediated IL-8 and IL-6 production by interacting with IL1RL1. TMED1 Protein, Human (HEK293, His) is the recombinant human-derived TMED1 protein, expressed by HEK293 , with C-10*His labeled tag.

TMED1 蛋白可能在囊泡蛋白运输中发挥作用，特别是在早期分泌途径中。它可以充当腔侧的货物受体，以促进分泌性货物分子掺入运输囊泡中，并且它还涉及细胞质侧的囊泡外壳形成。此外，TMED1 通过与白细胞介素 33 受体 IL1RL1 相互作用，对 IL-33 介导的 IL-8 和 IL-6 产生产生积极影响。此外，它还通过与 RNF26 的相互作用，通过 cGAS-STING 途径参与先天免疫信号的调节。TMED1 在内质网、内质网-高尔基体中间室和顺式高尔基体网络中形成同二聚体。它与 IL1RL1 和 RNF26 相互作用，后者的相互作用对于通过 cGAS-STING 途径调节先天免疫信号传导特别重要。

Immobilized Human TMED1 at 10 μg/mL (100 μL/well) can bind Biotinylated ST2 protein. The ED₅₀ for this effect is 79.64 ng/mL, corresponding to a specific activity is 1.26×10^4 Unit/mg.

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  Immobilized Human TMED1 at 10 μg/mL (100 μL/well) can bind Biotinylated ST2 protein. The ED₅₀ for this effect is 79.64 ng/mL, corresponding to a specific activity is 1.26×104 Unit/mg.

Human

HEK293

C-10*His

Q13445 (A24-N194)

11018  [NCBI]

Extracellular Domain

AGPPPIQDGEFTFLLPAGRKQCFYQSAPANASLETEYQVIGGAGLDVDFTLESPQGVLLVSESRKADGVHTVEPTEAGDYKLCFDNSFSTISEKLVFFELIFDSLQDDEEVEGWAEAVEPEEMLDVKMEDIKESIETMRTRLERSIQMLTLLRAFEARDRNLQEGNLERVN

Approximately 28 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.