UBE2T 蛋白, Human (His)

UBE2T protein is an important ubiquitination component. As an E2 ubiquitin-conjugating enzyme, it accepts ubiquitin in the E1 complex and catalyzes its covalent attachment to proteins. This multifaceted enzyme catalyzes monoubiquitination, which is critical during MMC-induced DNA repair. UBE2T Protein, Human (His) is the recombinant human-derived UBE2T protein, expressed by E. coli , with N-6*His labeled tag.

UBE2T 是泛素化机制的重要组成部分，作为 E2 泛素结合酶，接受 E1 复合物中的泛素并催化其与其他蛋白质的共价附着。这种多面酶具有催化单泛素化的能力，在丝裂霉素-C (MMC) 诱导的 DNA 修复过程中发挥着至关重要的作用。UBE2T 的重要性因其与范可尼贫血复合体的特定关联而得到强调，在范可尼贫血复合体中，它与 E3 泛素蛋白连接酶 FANCL 协同催化 FANCD2 的单泛素化，这是 DNA 损伤反应途径中不可或缺的一步。除了在 DNA 修复中的作用外，UBE2T 通过介导 FANCL 和 FANCI 的单泛素化并可能促进 BRCA1 的泛素化和降解而表现出多功能性。此外，体外研究揭示了 UBE2T 促进各种类型的多泛素化的能力，展示了其参与多种泛素依赖性过程。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

Q9NPD8 (M1-V197)

29089  [NCBI]

Full Length

MQRASRLKRELHMLATEPPPGITCWQDKDQMDDLRAQILGGANTPYEKGVFKLEVIIPERYPFEPPQIRFLTPIYHPNIDSAGRICLDVLKLPPKGAWRPSLNIATVLTSIQLLMSEPNPDDPLMADISSEFKYNKPAFLKNARQWTEKHARQKQKADEEEMLDNLPEAGDSRVHNSTQKRKASQLVGIEKKFHPDV

25-30 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 2 mM DTT, 10% glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.