1. 重组蛋白
  2. Ubiquitin Related Proteins
  3. Ubiquitin Enzymes
  4. E2 Enzymes
  5. Ubiquitin Conjugating Enzyme E2 V2
  6. UBE2V2 蛋白, Human (sf9, His, strep)

UBE2V2 蛋白, Human (sf9, His, strep)

目录号: HY-P704082
产品使用指南 技术支持

UBE2V2蛋白缺乏独立的泛素连接酶活性,并与UBE2N形成功能性异二聚体。它们共同催化非经典多聚泛素链合成(“Lys-63”),与蛋白酶体驱动的降解不同。UBE2V2 蛋白, Human (sf9, His, strep) 是重组的 UBE2V2 蛋白,由 Sf9 insect cells 表达,带有 N-His 和 N-Strep 标签。

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描述

UBE2V2 protein lacks independent ubiquitin ligase activity and forms a functional heterodimer with UBE2N. Together, they catalyze nonclassical polyubiquitin chain synthesis ("Lys-63"), distinct from proteasome-driven degradation. UBE2V2 Protein, Human (sf9, His, strep) is the recombinant human-derived UBE2V2 protein, expressed by Sf9 insect cells, with N-His and N-Strep labeled tag.

研究背景

UBE2V2 蛋白单独作用时缺乏泛素连接酶活性,但与 UBE2N 形成功能性异二聚体,催化通过“Lys-63”连接的非规范多泛素链的合成。值得注意的是,这种类型的多聚泛素化不会导致蛋白酶体降解蛋白质。UBE2V2 在介导靶基因的转录激活、影响细胞周期进程以及促进细胞分化方面发挥着关键作用。此外,它还参与无错误的 DNA 修复途径,增强 DNA 损伤后的细胞存活率。UBE2V2 主要作为 UBE2N 的异二聚体发挥作用,并表现出与 CHFR 的结合亲和力,表明其参与各种细胞过程和分子相互作用。

种属

Human

表达系统

Sf9 insect cells

标签

N-His;N-Strep

蛋白编号

Q15819 (M1-N145)

基因 ID

7336

蛋白结构
N-term
His
Strep
UBE2V2 (M1-N145)
Accession # Q15819
C-term
蛋白长度

Full Length

同用名
Ubiquitin-Conjugating Enzyme E2 Variant 2; DDVit 1; Enterocyte Differentiation-Associated Factor 1; EDAF-1; Enterocyte Differentiation-Promoting Factor 1; EDPF-1; MMS2 Homolog; Vitamin D3-Inducible Protein; UBE2V2; MMS2; UEV2
氨基酸序列

MAVSTGVKVPRNFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWTGMIIGPPRTNYENRIYSLKVECGPKYPEAPPSVRFVTKINMNGINNSSGMVDARSIPVLAKWQNSYSIKVVLQELRRLMMSKENMKLPQPPEGQTYNN

预测分子量
16.4 kDa
分子量

Approximately 16.4 kDa,based on SDS-PAGE under reducing conditions.

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

UBE2V2 Protein, Human (sf9, His, strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
UBE2V2 Protein, Human (sf9, His, strep)
目录号:
HY-P704082
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