2. Metabolic Enzyme/Protease Apoptosis Immunology/Inflammation Anti-infection
  3. Ser/Thr Protease Apoptosis Interleukin Related Bacterial
  4. Subtilisin

Subtilisin  (Synonyms: 枯草杆菌蛋白酶; EC 3.4.21.14)

目录号: HY-P2729
COA 产品使用指南 技术支持

Subtilisin (EC 3.4.21.14) 是一种细菌丝氨酸蛋白酶 (bacterial serine protease)。Subtilisin 可诱导凋亡 (Apoptosis)。Subtilisin 可刺激促过敏细胞因子 (IL-1α、IL-33) 表达。Subtilisin 可诱导原型过敏性肺部炎症。Subtilisin 对乳腺癌和结肠癌具有抗癌活性。Subtilisin 具有防污活性。Subtilisin 可作为洗涤剂添加剂。

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Subtilisin Chemical Structure

Subtilisin Chemical Structure

CAS No. : 9014-01-1

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Subtilisin 相关抗体

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查看 Interleukin Related 亚型特异性产品:

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IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Subtilisin (EC 3.4.21.14) is a bacterial serine protease. Subtilisin induces Apoptosis. Subtilisin stimulates the expression of pro-allergic cytokines (IL-1α, IL-33). Subtilisin induces prototypic allergic lung inflammation. Subtilisin exhibits anticancer activity against breast and colon cancer. Subtilisin shows antifouling activity. Subtilisin can be used as a detergent additive[1][2][3][4][5][6].

体外研究
(In Vitro)

产品信息
是经原生质体诱变方法选育的枯草杆菌通过深层发酵、提取及精制而成的一种蛋白水解酶。能水解蛋白质分子肽链生成多肽或氨基酸，在有机溶剂中还可、催化多肽的合成，本品含有稳定剂。

有效温度范围：20-60℃，最适作用温度在 35-45℃ 之间。
有效 pH 范围：6.0-11.0，最适 pH 值范围在 9.5-10.5 之间。
激活剂：Ca2+、Mg2+、Mn2+、Zn2+、Fe2+。
抑制剂：作用于丝氨酸的试剂如磺酰卤化物。

使用说明
可溶于 100 mg/mL ddH2O，避免反复冻融。或根据具体实验参考文献溶解。
Subtilisin (3-5 μg/mL；24 h) 对 MCF-7 和 HT-29 癌细胞表现出细胞毒作用，MCF-7 细胞表现出凋亡细胞特征，HT-29 细胞表现出坏死细胞特征[1]。
Subtilisin 具有防污活性[2]。
Subtilisin (1.0-10 μg/mL；3-6 h) 可刺激 H292 细胞中促过敏细胞因子 IL-1α、IL-33、TSLP 和生长因子双调蛋白的表达[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Subtilisin 相关抗体:

Apoptosis Analysis[1]

Cell Line: MCF-7
Concentration: 0 μg/mL, 3 μg/mL, 5 μg/mL
Incubation Time: 24 h
Result: Increased apoptotic rate.
Showed no changes in the levels of pro-apoptotic protein Bax and anti-apoptotic protein Bcl-2.
体内研究
(In Vivo)

Subtilisin (1 μg；皮下或鼻内注射；致敏两次，激发两次；整个实验周期为 22 天) 可在 C57BL/6 小鼠中诱发原发性过敏性肺部炎症[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female C57BL/6 mice (8-12 weeks old), allergic airway disease model[4]
Dosage: 1 μg
Administration: Subcutaneous (s.c.) or intranasal (i.n.). Sensitization: s.c. or i.n. injection with 1 μg of subtilisin on days 0 and 7. Challenge: i.n. administration with 1 μg of subtilisin on days 14 and 21. whole experimental cycle of 22 days
Result: Induced high levels of serum IgE and specific IgG1.
Led to intense airway eosinophilic inflammation, as shown by total and differential cell counts in bronchoalveolar lavage (BAL).
Induced pronounced airway inflammation and mucus formation.
Promoted increased bronchoconstriction to inhaled methacholine and induced a late-phase response.
Induced allergic responses dependent on its protease activity, protease-activated receptor (PAR)-2, IL-33 receptor ST2, and MyD88 signaling.
CAS 号
性状

固体

颜色

Light yellow to light brown

中文名称

枯草杆菌蛋白酶
EC 号

3.4.21.62
酶活

≥150 U/mg soild
单位定义

One unit is defined as the amount of enzyme that can hydrolyze casein to produce 1 μg of tyrosine per min at pH 10 and 40°C
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
溶解性数据
细胞实验： 

H2O 中的溶解度 : ≥ 100 mg/mL

* "≥" means soluble, but saturation unknown.

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
纯度 & 产品资料
参考文献

Subtilisin 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Subtilisin9014-01-1EC 3.4.21.14枯草杆菌蛋白酶Ser/Thr ProteaseApoptosisInterleukin RelatedBacterialSerine proteasesSerine endopeptidasesThreonine proteasesILC57BL/6lung inflammationMCF-7HT-29pro-allergicanticancerInhibitorinhibitorinhibit

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