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  3. Trypsin/Lys-C complex protease (MS grade)

Trypsin/Lys-C complex protease (MS grade)  (Synonyms: Trypsin/Lys-C复合蛋白酶)

目录号: HY-E70574
产品使用指南 技术支持

Trypsin/Lys-C complex protease (MS grade) 将Trypsin 和 Lys-C 两种重组蛋白酶结合在一起,以实现高效的肽键水解。Trypsin 特异性裂解精氨酸 (R) 和赖氨酸 (K) 的 C 端肽键,而 Lys-C 特异性裂解赖氨酸 (K) 的 C 端肽键。这种组合克服了由于 Trypsin 对赖氨酸和精氨酸的消化速度较慢、赖氨酸上的 PTM 改变或疏水性 C 末端(例如脯氨酸)而导致的切割遗漏等问题。Trypsin/Lys-C complex protease (MS grade) 可用于处理难酶解消化的复杂蛋白样品。用于蛋白表征,单细胞组学和大队列蛋白组学研究。

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Trypsin/Lys-C complex protease (MS grade) Chemical Structure

Trypsin/Lys-C complex protease (MS grade) Chemical Structure

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
20 μg ¥1280
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  • 生物活性

  • 纯度 & 产品资料

生物活性

Trypsin/Lys-C complex protease (MS grade) combines Trypsin and Lys-C, two recombinant proteases, to achieve efficient peptide bond hydrolysis. Trypsin specifically cleaves the C-terminal peptide bonds of arginine (R) and lysine (K), while Lys-C specifically cleaves the C-terminal peptide bonds of lysine (K). This combination overcomes issues such as the slower digestion rate of lysine and arginine by rTrypsin, PTM changes on lysine, or hydrophobic C-termini (such as proline) that can lead to missed cleavage. Trypsin/Lys-C complex protease (MS grade) can be used to process complex protein samples that are difficult to enzymatically digest. Trypsin/Lys-C complex protease (MS grade) can be used for protein characterization, single-cell proteomics and large cohort proteomics studies.

中文名称

Trypsin/Lys-C复合蛋白酶

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料

Trypsin/Lys-C complex protease (MS grade) 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Trypsin/Lys-C complex protease (MS grade)
目录号:
HY-E70574
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