Cytochrome P450 2C9 抗体 (YA2166)

Cytochrome P450 2C9 Antibody (YA2166) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2166), targeting Cytochrome P450 2C9, with a predicted molecular weight of 56 kDa (observed band size: 50 kDa). Cytochrome P450 2C9 Antibody (YA2166) can be used for WB, ICC/IF, FC experiment in human background.

Cytochrome P450 2C9 是一种细胞色素 P450 单加氧酶，参与多种内源性底物的代谢，包括脂肪酸和类固醇。其作用机制是利用分子氧，将一个氧原子插入底物，并将第二个氧原子还原为水分子，同时通过细胞色素 P450 还原酶 (NADPH--hemoprotein reductase) 从 NADPH 获取两个电子。该酶催化多不饱和脂肪酸 (PUFA) 双键的环氧化反应，以及碳氢键的羟基化反应。在胆固醇代谢中，Cytochrome P450 2C9 催化胆固醇转化为 25-羟基胆固醇，后者是细胞胆固醇稳态的生理调节剂。此外，该酶对 17β-雌二醇 (E2) 和雌酮 (E1) 形成儿茶酚雌激素 (如 2-羟基 E1 和 E2) 的催化活性较低。它还能催化多不饱和脂肪酸 (PUFA) 的双烯丙位羟基化反应及双键迁移羟基化反应。类似功能还包括代谢植物单萜类化合物，如柠檬烯，将其氧化为香芹醇和紫苏醇。Cytochrome P450 2C9 还影响多种药物的药代动力学变异性，如 S-华法林、双氯芬酸、苯妥英、甲苯磺丁脲和氯沙坦。

P11712

Predicted band size: 56 kDa; Observed band size: 50 kDa

Affinity Chromatography

Non-conjugated

Unmodified

AB_3104530

Cardiovascular

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1/500-1/1000;IF: 1/50-1/200;FC: 1/50-1/100

• 
  Western blot analysis was performed on extracts from HepG2 (lane 1, 20 μg), using CYP2C9 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, ICC/IF, FC

液体

Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human Cytochrome P450 2C9