PAK2 抗体 (YA695)

PAK2 Antibody (YA695) is a non-conjugated and Mouse origined monoclonal antibody about 58 kDa, targeting to PAK2 (7H3). It can be used for WB,IHC-F,IHC-P,ICC/IF assays with tag free, in the background of Human, Monkey.

PAK2 (7H3)：p21 活化激酶 (PAK) 是将 Rho GTP 酶与细胞骨架重组和核信号转导联系起来的关键效应物。PAK 蛋白是一个丝氨酸/苏氨酸激酶家族，可作为小 GTP 结合蛋白、CDC42 和 RAC1 的靶标，并涉及广泛的生物活性。由该基因编码的蛋白质在半胱天冬酶介导的细胞凋亡过程中被蛋白水解切割激活，并可能在调节垂死细胞的细胞凋亡事件中发挥作用。[由 RefSeq 提供，2008 年 7 月]

Free

5062

Predicted band size: 58 kDa

affinity purified

Non-conjugated

Cell Biology

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Monkey

Q13177

WB: 1:500-1:1,000 ;IHC: 1:50-1:100 ;IF: 1:50-1:200

• 
  Western blot analysis of extracts from Jurkat(lane 2(20μg))、MCF-7 (lane 3(20μg) ) and Hela (lane 4(20μg)), using PAK2 (HY-P80778) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80778, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IHC-F, IHC-P, ICC/IF

液体

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Synthetic peptide corresponding tofragment of PAK2.The exact sequence is proprietary to MCE.