Phospho-CDK1/2 (Thr14) 抗体 (YA2572)

Phospho-CDK1/2 (Thr14) Antibody (YA2572) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2572), targeting Phospho-CDK1/2 (Thr14), with a predicted molecular weight of 34 kDa (observed band size: 34 kDa). Phospho-CDK1/2 (Thr14) Antibody (YA2572) can be used for WB, IHC-P, IP experiment in human background.

CDC2 是一种关键蛋白，通过调节中心体周期和有丝分裂起始在真核细胞周期调控中发挥核心作用。它通过与多种间期 cyclins 结合促进 G2-M 期转换，并磷酸化包括 PARVA/actopaxin、APC、BARD1 等在内的众多底物，参与中心体分离、高尔基体动态、核膜破裂和染色体凝聚等过程。CDC2 还调控纺锤体形成、微管动力学及细胞凋亡相关信号（如磷酸化 Bcl-xL/BCL2L1 触发凋亡，而磷酸化 CASP8 则抑制其活化）。在 DNA 损伤时，CDC2 活性被抑制以实现 G2 期检查点阻滞；修复后通过 WIP1 信号重新激活。此外，CDC2 磷酸化核心时钟基因调控因子 NR1D1 影响生物钟振幅，并在有丝分裂中抑制 CGAS 以防止自身 DNA 激活免疫应答。类似功能包括磷酸化 KRT5（by similar）和 CALD1（by similar）以分别参与中期进程和雪旺细胞迁移。CDC2 还可作为丙型肝炎病毒（HCV）进入肝细胞的受体。

P06493

Predicted band size: 34 kDa; Observed band size: 34 kDa

Affinity Purified

Non-conjugated

Phosphorylated

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1/500-1/1000;IHC: 1/50-1/100;IP: 1/20

WB, IHC-P, IP

液体

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthetic phosphopeptide corresponding to residues surrounding Thr14 of human Cdk1/2