1. 抗体
  2. 一抗
  3. 多克隆抗体
  4. PKM2 抗体

PKM2 Antibody 是一个非偶联、分子量约 58 kDa、兔源、抗 PKM2 多克隆抗体。它可用于人、小鼠、大鼠背景下 WB,IHC-P,ICC/IF,FC实验,且不带标记。

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规格 价格 是否有货 数量
10 μL ¥470 In-stock
50 μL ¥1220 In-stock
100 μL ¥2000 In-stock
250 μL   询价  

* Please select Quantity before adding items.

【WB: 蛋白质免疫印迹; IHC-P: 石蜡切片样本的免疫组织化学; IHC-F: 冰冻切片样本的免疫组织化学; ICC/IF: 细胞免疫荧光; IF-Tissue: 组织免疫荧光; mIHC: 多重荧光免疫组化; IP: 免疫沉淀; ChIP: 染色质免疫沉淀; FC: 流式细胞术; ELISA: 酶联免疫吸附试验】

  • 生物活性

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描述

PKM2 Antibody is a non-conjugated and Rabbit origined polyclonal antibody about 58 kDa, targeting to PKM2. It can be used for WB,IHC-P,ICC/IF,FC assays with tag free, in the background of Human, Mouse, Rat.

研究背景

PKM2:该基因编码参与糖酵解的蛋白质。编码的蛋白质是一种丙酮酸激酶,可催化磷酸基团从磷酸烯醇丙酮酸转移到 ADP,生成 ATP 和丙酮酸。这种蛋白质已被证明与甲状腺激素相互作用,并可能介导甲状腺激素诱导的细胞代谢效应。已发现该蛋白与 Opa 蛋白结合,Opa 蛋白是一种参与淋球菌粘附和侵入人体细胞的细菌外膜蛋白,表明该蛋白在细菌发病机制中的作用。已经报道了几种编码几种不同亚型的可变剪接转录物变体。[由 RefSeq 提供,2011 年 5 月]

标签

Free

基因 ID
蛋白数据库
中文名
PKM2 抗体
同用名
PKM; OIP3; PK2; PK3; PKM2; Pyruvate kinase isozymes M1/M2; Cytosolic thyroid hormone-binding protein; CTHBP; Opa-interacting protein 3; OIP-3; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; THBP1; Tu
分子量

Predicted band size: 58 kDa

纯度

affinity purified

偶联

Non-conjugated

修饰

Unmodified

RRID
研究领域

Signal Transduction

产品类别

Primary Antibody; Rabbit Polyclonal Antibody

克隆性

Polyclonal

宿主

Rabbit

反应物种

Human, Mouse, Rat

推荐稀释比例

WB: 1/500-1/1000;IHC-P:1/50-1/200;ICC/IF:1/50-1/200;FC:1/50

  • Western blot analysis of extracts from C6(lane 2(20μg) ,NIH/3T3(lane 3(20μg) and Hela(lane 4(20ug)using PKM2 Antibody (HY-P80870) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using PKM2 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using PKM2 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
应用

WB, IHC-P, ICC/IF, FC

性状

液体

组分

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

同型

IgG

敏感性

Endogenous

免疫原

Synthetic peptide corresponding to Human PKM2 AA range 50-100.

数据库

Entrez Gene: 5315 Human ;

SwissProt: P14618 Human ;

OMIM: 179050 Human

文件资料

PKM2 Antibody 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Inquiry Information

产品名称:
PKM2 Antibody
目录号:
HY-P80870
需求量: