PKM2 抗体

PKM2 Antibody is a non-conjugated and Rabbit origined polyclonal antibody about 58 kDa, targeting to PKM2. It can be used for WB,IHC-P,ICC/IF,FC assays with tag free, in the background of Human, Mouse, Rat.

PKM2：该基因编码参与糖酵解的蛋白质。编码的蛋白质是一种丙酮酸激酶，可催化磷酸基团从磷酸烯醇丙酮酸转移到 ADP，生成 ATP 和丙酮酸。这种蛋白质已被证明与甲状腺激素相互作用，并可能介导甲状腺激素诱导的细胞代谢效应。已发现该蛋白与 Opa 蛋白结合，Opa 蛋白是一种参与淋球菌粘附和侵入人体细胞的细菌外膜蛋白，表明该蛋白在细菌发病机制中的作用。已经报道了几种编码几种不同亚型的可变剪接转录物变体。[由 RefSeq 提供，2011 年 5 月]

Free

P14618

Predicted band size: 58 kDa; Observed band size: 58 kDa

affinity purified

Non-conjugated

Unmodified

AB_3102733

Signal Transduction

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse, Rat

WB: 1/500-1/1000；IHC-P：1/50-1/200；ICC/IF：1/50-1/200；FC：1/50

• 
  Western blot analysis of extracts from C6(lane 2(20μg) ,NIH/3T3(lane 3(20μg) and Hela(lane 4(20ug)using PKM2 Antibody (HY-P80870) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded mouse testis tissue using PKM2 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse testis tissue using PKM2 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF, FC

液体

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human PKM2 AA range 50-100.