1. Academic Validation
  2. Effect of vascular bradykinin on pancreatic microcirculation and hemorheology in rats with severe acute pancreatitis

Effect of vascular bradykinin on pancreatic microcirculation and hemorheology in rats with severe acute pancreatitis

  • Eur Rev Med Pharmacol Sci. 2015;19(14):2646-50.
L-T Liu 1 Y Li L-Q Fan Q Zhao D Wang S-J Cheng A-M Zhang Y Qin B Zhang
Affiliations

Affiliation

  • 1 Department of General Surgery, The No. 4 Affiliated Hospital of Hebei Medical University, Shijiazhuang, China. yonglicn@yeah.net.
PMID: 26221896
Abstract

Objective: To investigate the effect of vascular bradykinin on pancreatic microcirculation and hemorheology in rats with severe acute pancreatitis (SAP).

Materials and methods: Ninety male Wistar rats were randomly divided into a blank control group, an SAP group and a vascular bradykinin treatment group. The SAP model was induced by the retrograde injection of 5% sodium taurocholate in the pancreaticobiliary duct. The vascular bradykinin treatment group underwent gastrostomy, with a fine plastic tube placed in the stomach that led out of body through the abdominal wall.Vascular bradykinin was fully dissolved and administered at a dose of 20 U/kg once every 8 h. The pancreatic microcirculatory blood flow volume and velocity, microvascular permeability, hemorheology were evaluated respectively by double-channel laser Doppler flowmetry, the Evans blue leakage test, a blood rheology test instrument.

Results: The pancreatic microcirculatory blood flow volume and velocity in the vascular bradykinin treatment group increased gradually after 48 h compared with the SAP group, and the changes were significantly different (p < 0.05). The pancreatic microvascular permeability of the vascular bradykinin treatment group was significantly reduced after 48 h compared with the SAP group (p < 0.05). The low shear rate blood viscosity, hematocrit and erythrocyte aggregation index of the vascular bradykinin treatment group were significantly decreased after 48 h compared with the SAP group (p < 0.05).

Conclusions: Vascular bradykinin can improve pancreatic microcirculation and hemorheology in rats with severe acute pancreatitis.

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