2. Immunology/Inflammation Apoptosis
  3. Toll-like Receptor (TLR) Apoptosis
  4. Polyinosinic-polycytidylic acid potassium

Polyinosinic-polycytidylic acid potassium  (Synonyms: Poly(I:C) potassium)

目录号: HY-134958
产品使用指南 技术支持

Polyinosinic-polycytidylic acid potassium (Poly(I:C) potassium) 是双链 RNA 的合成类似物，是一种 TLR3 和视黄酸诱导型基因 I 受体 (RIG-I 和 MDA5) 的激动剂。Polyinosinic-polycytidylic acid potassium 可以用作疫苗佐剂，以增强先天性和适应性免疫反应，并改变肿瘤的微环境，还可以直接触发癌细胞发生凋亡 (Apoptosis)。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

Polyinosinic-polycytidylic acid potassium Chemical Structure

Polyinosinic-polycytidylic acid potassium Chemical Structure

CAS No. : 31852-29-6

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货
1 mg ￥1281
询问价格 & 货期
5 mg ￥3202
询问价格 & 货期
10 mg ￥5124
询问价格 & 货期
25 mg 现货 询问价格 & 货期
50 mg 现货 询问价格 & 货期

* Please select Quantity before adding items.

Polyinosinic-polycytidylic acid potassium 的其他形式现货产品:

Customer Review

Other Forms of Polyinosinic-polycytidylic acid potassium:

Polyinosinic-polycytidylic acid potassium 相关抗体

Top Publications Citing Use of Products

    Polyinosinic-polycytidylic acid potassium purchased from MCE. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    BECN1 knockout HeLa cells were transfected with pCAGGS, HA-8A or HA-NSs for 24 h, and then transfected with poly(I:C) for 24 h. IFNB, IFIT1 and CXCL10 mRNA levels were analyzed with RT-qPCR.

    Polyinosinic-polycytidylic acid potassium purchased from MCE. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    HeLa cells were transfected with pCAGGS, HA-8A or HA-NSs at a dose of 2 μg for 24 h, and then transfected with poly(I:C) for 24 h. IFNB, IFIT1 and CXCL10 mRNA levels were analyzed with RT-qPCR.

    Polyinosinic-polycytidylic acid potassium purchased from MCE. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    HEK293T cells were transfected with HA-8A or HA-NSs at a dose of 2 μg for 24 h, and then transfected with poly(I:C) for 24 h. Co-IP with HA antibodies and evaluation of LC3, TBK1 and TRIM25 with western blot using IgG as control.

    Polyinosinic-polycytidylic acid potassium purchased from MCE. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    HEK293T cells were transfected with pCAGGS, HA-NSs and HA-8A, HA-PxxP, HA-DWP, or HA-WxxL at a dose of 2 μg for 24 h, and then transfected with poly(I:C) for 24 h. IFNB and IFIT1 mRNA levels were analyzed with RT-qPCR.

    Polyinosinic-polycytidylic acid potassium purchased from MCE. Usage Cited in: Chem Eng J. 2021 Aug 15;418:129392.

    Immunofluorescence staining photographs of lung sections. Scale bars, 100 μm. (I: PBS, II: Poly(I:C), III: PL-Poly(I:C), IV: NL-Poly(I:C), V: PL-Poly(I:C)/RBD, VI: NL-Poly(I:C)/RBD).

    查看 Toll-like Receptor (TLR) 亚型特异性产品:

    查看所有亚型
    TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Polyinosinic-polycytidylic acid potassium (Poly(I:C) potassium) is a synthetic analog of double-stranded RNA and an agonist of toll-like receptor 3 (TLR3) and retinoic acid inducible gene I (RIG-I)-like receptors (RIG-I and MDA5). Polyinosinic-polycytidylic acid sodium can be used as a vaccine adjuvant to enhance innate and adaptive immune responses, and to alter the tumor microenvironment. Polyinosinic-polycytidylic acid potassium can directly trigger cancer cells to undergoApoptosis[1][2][3].

    IC50 & Target[1]

    TLR3

     

    体外研究
    (In Vitro)

    Polyinosinic-polycytidylic acid potassium (20 ng/mL; 24 hours; WM793, WM278, WM239A, WM9 and 1205Lu cells) 抑制细胞生长活性[1].
    Polyinosinic-polycytidylic acid potassium (200 ng/mL; 24 hours; 1205Lu cells) 诱导 1205Lu 细胞凋亡[1]。
    Polyinosinic-polycytidylic acid potassium (5 ng/mL; 24 hours; 1205Lu cells) 诱导黑素瘤细胞中的 IFN-β 表达。RIG-I 和 MDA-5 的沉默证实 Polyinosinic-polycytidylic acid potassium 诱导 IFN-β 分别需要 RIG-I 和 MDA-5，并且需要 IPS-1[1]。
    Polyinosinic-polycytidylic acid potassium 重悬于无菌盐水中，以 2 mg/ml 的浓度加热至 50 °C 以确保完全溶解，然后自然冷却至室温以确保双链 RNA 的适当退火，从而制备用于注射的 Polyinosinic-polycytidylic acid potassium. Poly I:C 储存在 -20 °C 直至使用[3]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Polyinosinic-polycytidylic acid potassium 相关抗体:

    Cell Viability Assay[1]

    Cell Line: WM793, WM278, WM239A, WM9 and 1205Lu cells
    Concentration: 20 ng/mL
    Incubation Time: 24 hours
    Result: Strongly reduced viability from 100% in controls to 20%–50% within 24 hours.

    Apoptosis Analysis[1]

    Cell Line: 1205Lu cells
    Concentration: 200 ng/mL
    Incubation Time: 24 hours
    Result: Induced apoptosis in 1205Lu cells.

    RT-PCR[1]

    Cell Line: WM793, WM278, WM239A, WM9 and 1205Lu cells
    Concentration: 3 ng/mL
    Incubation Time: 24 hours
    Result: Induced IFN-β expression in melanoma cells.

    Western Blot Analysis[1]

    Cell Line: 1205Lu cells
    Concentration: 5 ng/mL
    Incubation Time: 24 hours
    Result: Revealed active subunits of caspase-9 and caspase-8 in melanoma cells.
    体内研究
    (In Vivo)

    Polyinosinic-polycytidylic acid potassium (50 µg; i.v.; on days 3, 6, and 9 after tumor inoculation) 在 NOD/SCID 免疫缺陷小鼠中显示出抗肿瘤活性[1]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: NOD/SCID immunodeficient mice (1205Lu cells)[1]
    Dosage: 50 µg
    Administration: I.v.; on days 3, 6, and 9 after tumor inoculation
    Result: Reduced the size of metastases and the total amount of tumor tissue, and the level of human DNA was 50% lower in mice.
    同用名

    Poly(I:C) potassium

    Formula

    (C10H13N4O8P)x.(C9H14N3O8P)x.xK
    CAS 号
    中文名称

    卡那霉素-聚肌胞钾盐复合物
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    Please store the product under the recommended conditions in the Certificate of Analysis.
    纯度 & 产品资料
    参考文献

    Polyinosinic-polycytidylic acid potassium 相关分类

    • 摩尔计算器

    • 稀释计算器

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量   浓度   体积   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Polyinosinic-polycytidylic acid31852-29-6Poly(I:C)卡那霉素-聚肌胞钾盐复合物Toll-like Receptor (TLR)ApoptosisTLR3RIG-IMDA5immunotherapytumormicroenvironment apoptosisdsRNAproperannealingdouble-strandedRNAInhibitorinhibitorinhibit

    您最近查看的产品:

    Your information is safe with us. * Required Fields.

       产品名称：

    		  

    * 需求量：

    * 客户姓名：

    		 

    * Email：

    * 电话：

    		 

    * 公司或机构名称：

       留言给我们：

    Bulk Inquiry

    Inquiry Information

    产品名称：
    Polyinosinic-polycytidylic acid potassium
    目录号：
    HY-134958
    需求量：

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

       产品名称：

    		  

    * Lot #：

    * 客户姓名：

    		 

    * Email：

       电话：

    		 

    * 部门：

    * 公司或机构名称：

    		 

       留言给我们：

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.

    嗨！很高兴为您提供帮助！

    尊敬的 MCE 客户您好，
    请您选择所在区域，我们将转接对应客服为您服务！

    热门区域

    A

    B

    C

    F

    G

    H

    J

    L

    N

    Q

    S

    T

    X

    Y

    Z

    热 A B C F G H J L N Q S T X Y Z