2. Immunology/Inflammation Apoptosis
  3. Toll-like Receptor (TLR) Apoptosis
  4. Polyinosinic-polycytidylic acid potassium

Polyinosinic-polycytidylic acid potassium  (Synonyms: Poly(I:C) potassium)

目录号: HY-134958
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摩尔计算器

Polyinosinic-polycytidylic acid potassium (Poly(I:C) potassium) 是双链 RNA 的合成类似物，是一种 TLR3 和视黄酸诱导型基因 I 受体 (RIG-I 和 MDA5) 的激动剂。Polyinosinic-polycytidylic acid potassium 可以用作疫苗佐剂，以增强先天性和适应性免疫反应，并改变肿瘤的微环境，还可以直接触发癌细胞发生凋亡 (Apoptosis)。

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Polyinosinic-polycytidylic acid potassium Chemical Structure

Polyinosinic-polycytidylic acid potassium Chemical Structure

CAS No. : 31852-29-6

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Polyinosinic-polycytidylic acid potassium 的其他形式现货产品:

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Other Forms of Polyinosinic-polycytidylic acid potassium:

Polyinosinic-polycytidylic acid potassium 相关抗体

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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Polyinosinic-polycytidylic acid potassium (Poly(I:C) potassium) is a synthetic analog of double-stranded RNA and an agonist of toll-like receptor 3 (TLR3) and retinoic acid inducible gene I (RIG-I)-like receptors (RIG-I and MDA5). Polyinosinic-polycytidylic acid sodium can be used as a vaccine adjuvant to enhance innate and adaptive immune responses, and to alter the tumor microenvironment. Polyinosinic-polycytidylic acid potassium can directly trigger cancer cells to undergoApoptosis[1][2][3].

IC50 & Target[1]

TLR3

 

体外研究
(In Vitro)

Polyinosinic-polycytidylic acid potassium (20 ng/mL; 24 hours; WM793, WM278, WM239A, WM9 and 1205Lu cells) 抑制细胞生长活性[1].
Polyinosinic-polycytidylic acid potassium (200 ng/mL; 24 hours; 1205Lu cells) 诱导 1205Lu 细胞凋亡[1]。
Polyinosinic-polycytidylic acid potassium (5 ng/mL; 24 hours; 1205Lu cells) 诱导黑素瘤细胞中的 IFN-β 表达。RIG-I 和 MDA-5 的沉默证实 Polyinosinic-polycytidylic acid potassium 诱导 IFN-β 分别需要 RIG-I 和 MDA-5，并且需要 IPS-1[1]。
Polyinosinic-polycytidylic acid potassium 重悬于无菌盐水中，以 2 mg/ml 的浓度加热至 50 °C 以确保完全溶解，然后自然冷却至室温以确保双链 RNA 的适当退火，从而制备用于注射的 Polyinosinic-polycytidylic acid potassium. Poly I:C 储存在 -20 °C 直至使用[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Polyinosinic-polycytidylic acid potassium 相关抗体:

Cell Viability Assay[1]

Cell Line: WM793, WM278, WM239A, WM9 and 1205Lu cells
Concentration: 20 ng/mL
Incubation Time: 24 hours
Result: Strongly reduced viability from 100% in controls to 20%–50% within 24 hours.

Apoptosis Analysis[1]

Cell Line: 1205Lu cells
Concentration: 200 ng/mL
Incubation Time: 24 hours
Result: Induced apoptosis in 1205Lu cells.

RT-PCR[1]

Cell Line: WM793, WM278, WM239A, WM9 and 1205Lu cells
Concentration: 3 ng/mL
Incubation Time: 24 hours
Result: Induced IFN-β expression in melanoma cells.

Western Blot Analysis[1]

Cell Line: 1205Lu cells
Concentration: 5 ng/mL
Incubation Time: 24 hours
Result: Revealed active subunits of caspase-9 and caspase-8 in melanoma cells.
体内研究
(In Vivo)

Polyinosinic-polycytidylic acid potassium (50 µg; i.v.; on days 3, 6, and 9 after tumor inoculation) 在 NOD/SCID 免疫缺陷小鼠中显示出抗肿瘤活性[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: NOD/SCID immunodeficient mice (1205Lu cells)[1]
Dosage: 50 µg
Administration: I.v.; on days 3, 6, and 9 after tumor inoculation
Result: Reduced the size of metastases and the total amount of tumor tissue, and the level of human DNA was 50% lower in mice.
Formula

(C10H13N4O8P)x.(C9H14N3O8P)x.xK
CAS 号
中文名称

卡那霉素-聚肌胞钾盐复合物
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

Polyinosinic-polycytidylic acid potassium 相关分类

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Polyinosinic-polycytidylic acid31852-29-6Poly(I:C)卡那霉素-聚肌胞钾盐复合物Toll-like Receptor (TLR)ApoptosisTLR3RIG-IMDA5immunotherapytumormicroenvironment apoptosisdsRNAproperannealingdouble-strandedRNAInhibitorinhibitorinhibit

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