PRCP 蛋白, Human (HEK293, His)

PRCP protein acts as an enzyme that cleaves the C-terminal amino acid linked to proline in peptides, including angiotensin II, III and des-Arg9-bradykinin. Although some substrates retain enzymatic activity even at neutral pH, this cleavage activity is noteworthy because it occurs at acidic pH. PRCP Protein, Human (HEK293, His) is the recombinant human-derived PRCP protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PRCP Protein, Human (HEK293, His) is 475 a.a., with molecular weight of 60-90 kDa.

PRCP（即脯氨酰羧肽酶）是一种酶，因其在选择性切割血管紧张素 II、III 和 des-Arg9-缓激肽等肽中与脯氨酸相连的 C 端氨基酸方面的作用而闻名。PRCP 的一个显着特征是其在裂解过程中偏爱酸性 pH 值。然而，即使在中性 pH 条件下，它仍保留特定底物的酶活性。这种双重 pH 响应性意味着 PRCP 在不同细胞环境中具有多种功能，影响重要生理途径中涉及的肽的加工和生物活性，特别是与肾素-血管紧张素和激肽系统相关的肽。PRCP 在一系列 pH 水平下发挥作用的能力表明其在肽代谢和信号传导中发挥着复杂的调节作用。

Human

HEK293

C-6*His

P42785 (L22-H496)

5547  [NCBI]

LRPALRALGSLHLPTNPTSLPAVAKNYSVLYFQQKVDHFGFNTVKTFNQRYLVADKYWKKNGGSILFYTGNEGDIIWFCNNTGFMWDVAEELKAMLVFAEHRYYGESLPFGDNSFKDSRHLNFLTSEQALADFAELIKHLKRTIPGAENQPVIAIGGSYGGMLAAWFRMKYPHMVVGALAASAPIWQFEDLVPCGVFMKIVTTDFRKSGPHCSESIHRSWDAINRLSNTGSGLQWLTGALHLCSPLTSQDIQHLKDWISETWVNLAMVDYPYASNFLQPLPAWPIKVVCQYLKNPNVSDSLLLQNIFQALNVYYNYSGQVKCLNISETATSSLGTLGWSYQACTEVVMPFCTNGVDDMFEPHSWNLKELSDDCFQQWGVRPRPSWITTMYGGKNISSHTNIVFSNGELDPWSGGGVTKDITDTLVAVTISEGAHHLDLRTKNALDPMSVLLARSLEVRHMKNWIRDFYDSAGKQH

60-90 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filter solution of 20 mM NaAc-HAc, 150 mM NaCl, 10% Glycerol, pH4.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.