Frizzled-5 蛋白, Human (HEK293, His)

Frizzled-5 protein is a receptor for Wnt proteins (WNT2, WNT10B, WNT5A), which can activate WNT7A and promote the formation of synapses in neurons. It participates in the canonical Wnt/β-catenin pathway, activating disheveled proteins, inhibiting GSK-3 kinase, and triggering Wnt target genes. Frizzled-5 Protein, Human (HEK293, His) is the recombinant human-derived Frizzled-5 protein, expressed by HEK293 , with C-His labeled tag. The total length of Frizzled-5 Protein, Human (HEK293, His) is 141 a.a., with molecular weight of 25-30 kDa.

Frizzled-5 蛋白作为 Wnt 蛋白（包括 WNT2、WNT10B 和 WNT5A）的受体发挥作用，尽管它在体外不会激活 WNT2B 或 WNT4，并且体内环境可能因共表达的潜在差异而有所不同。在神经元中，Frizzled-5 激活 WNT7A 可促进突触形成。它积极参与经典的 Wnt/β-连环蛋白信号通路，导致蓬乱蛋白的激活、GSK-3 激酶的抑制、β-连环蛋白的核积累以及随后 Wnt 靶基因的激活。Frizzled-5 还可能参与涉及 PKC 和钙流的次级信号传导途径，考虑到 PKC 对 Wnt 介导的 GSK-3 激酶失活的明显要求，其与经典途径的整合仍不清楚。这种多功能受体可能在组织形态发生和分化组织中极性信息的转导和细胞间传递中发挥作用。此外，Frizzled-5 与卵黄囊血管生成和胎盘血管形成有关，其同二聚化是通过 FZ 结构域与不饱和脂肪酸分子的结合来促进的。值得注意的是，Frizzled-5 与 WNT2B、WNT7A 和 GOPC 相互作用，有助于其发挥多种细胞功能。

Human

HEK293

C-His

Q13467 (A27-P167)

7855  [NCBI]

25-30 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.