1. 重组蛋白
  2. Receptor Proteins
  3. G-Protein-Coupled Receptors (GPCRs)
  4. Frizzled
  5. Frizzled-9
  6. Frizzled-9 蛋白, Mouse (HEK293, mFc)

Frizzled-9 蛋白, Mouse (HEK293, mFc)

目录号: HY-P79115
COA 产品使用指南 技术支持

Frizzled-9 是一种 WNT2 受体,对于激活 β-catenin 通路至关重要,参与 Disheveled 蛋白激活、GSK-3 激酶抑制、核 β-catenin 积累和 Wnt 靶基因激活。除了经典信号传导之外,它还对神经肌肉接头组装中的乙酰胆碱受体聚集进行负调节,并通过调节细胞周期来影响神经祖细胞的活力。Frizzled-9 蛋白, Mouse (HEK293, mFc) 是重组的 Frizzled-9 蛋白,由 HEK293 表达,带有 C-mFc 标签。

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Frizzled-9 is a WNT2 receptor that is critical for activation of the β-catenin pathway, involved in Disheveled protein activation, GSK-3 kinase inhibition, nuclear β-catenin accumulation, and Wnt target gene activation. In addition to classical signaling, it negatively regulates acetylcholine receptor clustering in neuromuscular junction assembly and affects the viability of neural progenitor cells by regulating the cell cycle. Frizzled-9 Protein, Mouse (HEK293, mFc) is the recombinant mouse-derived Frizzled-9 protein, expressed by HEK293 , with C-mFc labeled tag.

研究背景

Frizzled-9 是 WNT2 的受体,是 β-连环蛋白经典信号通路中的关键角色,协调蓬乱蛋白的激活、GSK-3 激酶的抑制、β-连环蛋白的核积累以及随后的 Wnt 激活目标基因。除了在经典 Wnt 信号传导中的作用之外,Frizzled-9 还表现出多方面的功能。它通过 β-连环蛋白途径负向调节乙酰胆碱受体 (AChR) 的聚集,从而有助于神经肌肉接头 (NMJ) 的组装。在神经祖细胞 (NPC) 中,Frizzled-9 通过负向调节细胞周期停滞来影响细胞活力,从而抑制细胞凋亡过程。该受体参与海马发育包括调节神经母细胞增殖和凋亡细胞死亡。此外,Frizzled-9 在骨动力学中发挥着关键作用,通过 ISG15 介导的非经典 Wnt 信号传导调节骨形成,并通过非经典 Wnt 信号传导途径对骨再生产生积极影响。

生物活性

Measured by its ability to bind biotinylated mouse Wnt-3a in a funtional ELISA with an estimated Kd 2.263 nM.

  • Immobilized Frizzled-9 at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse Wnt3a protein. The Kd is 2.263 nM.
种属

Mouse

表达系统

HEK293

标签

C-mFc

蛋白编号

Q9R216 (L24-D186)

基因 ID
蛋白结构
N-term
Frizzled-9 (L24-D186)
Accession # Q9R216
mFc
C-term
蛋白长度

Partial

中文名
Frizzled-9 蛋白
同用名
Frizzled-9; Fzd9; Fz-9; mFz3; mFz9; CD349; Fzd3
氨基酸序列

LEIGRFDPERGRGPAPCQAMEIPMCRGIGYNLTRMPNLLGHTSQGEAAAQLAEFSPLVQYGCHSHLRFFLCSLYAPMCTDQVSTPIPACRPMCEQARLRCAPIMEQFNFGWPDSLDCARLPTRNDPHALCMEAPENATAGPTEPHKGLGMLPVAPRPARPPGD

分子量

52-61 kDa

糖基化修饰
Yes
纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 500 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Frizzled-9 Protein, Mouse (HEK293, mFc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Frizzled-9 Protein, Mouse (HEK293, mFc)
目录号:
HY-P79115
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