1. 重组蛋白
  2. Cytokines and Growth Factors
  3. CSF & Receptors
  4. GM-CSF
  5. GM-CSF 蛋白, Rat (CHO)

GM-CSF 蛋白, Rat (CHO)

目录号: HY-P7094
COA 产品使用指南

GM-CSF 蛋白, Rat (CHO)是造血生长因子和免疫调节剂。

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规格 价格 是否有货 数量
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2 μg ¥350 In-stock
10 μg ¥900 In-stock
50 μg ¥2500 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

GM-CSF Protein, Rat (CHO) is a hematopoietic growth factor and immune modulator.

研究背景

粒细胞巨噬细胞集落刺激因子(GM-CSF)是由多种细胞类型(包括 T 细胞、巨噬细胞、内皮细胞和成纤维细胞)在接受免疫刺激后产生的。它是一种重要的造血生长因子和免疫调节剂。GM-CSF 对各种循环白细胞的功能活动也有深远的影响。GM-CSF根据其浓度刺激多能祖细胞,最低剂量时巨噬细胞祖细胞的增殖,其次是粒细胞、红细胞、嗜酸性粒细胞、巨核细胞和多能祖细胞。在某些情况下,它还能刺激髓系白血病细胞的分化并控制嗜酸性粒细胞功能[1][2]GM-CSF 还增强成熟细胞的功能,例如中性粒细胞。在中性粒细胞中,GM-CSF 增强脱颗粒、氧和氮自由基离子的释放、吞噬作用并抑制细胞凋亡[3]GM-CSF 抑制在一些自身免疫性疾病动物模型中表现出显着的有益作用[4]GM-CSF 还具有许多促炎功能,最近的数据表明 GM-CSF 是 Th17 驱动的自身免疫性炎症的关键因素[5]

生物活性

1.The ED50 is <5 pg/mL as measured by FDC-P1 cells, corresponding to a specific activity of >2 × 108 units/mg.
2.Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 5.019 pg/mL, corresponding to a specific activity is 1.99×108 units/mg.

  • Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 5.019 pg/mL, corresponding to a specific activity is 1.99×108 units/mg.
种属

Rat

表达系统

CHO

标签

Tag Free

蛋白编号

P48750 (A18-K144)

基因 ID
蛋白结构
N-term
GM-CSF (A18-K144)
Accession # P48750
C-term
同用名
rRtGM-CSF; CSF-2; MGI-1GM; Pluripoietin-alpha; Molgramostin; Sargramostim
氨基酸序列

APTRSPNPVTRPWKHVDAIKEALSLLNDMRALENEKNEDVDIISNEFSIQRPTCVQTRLKLYKQGLRGNLTKLNGALTMIASHYQTNCPPTPETDCEIEVTTFEDFIKNLKGFLFDIPFDCWKPVQK

分子量

16-26 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.22 μm filtered solution of PBS or 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<0.2 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

GM-CSF Protein, Rat (CHO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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GM-CSF Protein, Rat (CHO)
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