PTK7 蛋白, Rat (HEK293, His)

The PTK7 protein is predicted to mediate cell adhesion molecule binding and play critical roles in a variety of cellular processes, including actin cytoskeleton reorganization and regulation of the canonical Wnt signaling pathway. It affects animal organ development, axis elongation, and epithelial morphogenesis. PTK7 Protein, Rat (HEK293, His) is the recombinant rat-derived PTK7 protein, expressed by HEK293 , with C-10*His labeled tag. The total length of PTK7 Protein, Rat (HEK293, His) is 673 a.a., with molecular weight of 95-130 kDa.

PTK7 蛋白预计在介导细胞粘附分子结合活性中发挥关键作用。据预测，它参与多种细胞过程，包括肌动蛋白细胞骨架的重组、细胞对视黄酸的反应以及经典 Wnt 信号通路的正向调节。此外，预计它会对动物器官发育、轴伸长和上皮形态发生等基本过程的上游或内部产生影响。PTK7 的预测亚细胞定位位于膜中，在细胞-细胞连接处和质膜上具有预期活性。值得注意的是，PTK7 表现出偏向表达，在肺 (RPKM 114.4)、子宫 (RPKM 97.1) 和其他八个组织中观察到水平升高，表明其在这些生理环境中的潜在意义。[信息来自基因组资源联盟，2022 年 4 月]

Measured by its binding ability in a functional ELISA. Recombinant Rat PTK7/CCK4 Fc Chimera binds Biotinylated Recombinant Mouse Wnt-3a. The ED₅₀ for this effect is 8.203 μg/mL.

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  Measured by its binding ability in a functional ELISA. Recombinant Rat PTK7/CCK4 Fc Chimera binds Biotinylated Recombinant Mouse Wnt-3a. The ED₅₀ for this effect is 8.203 μg/mL.

Rat

HEK293

C-10*His

NP_001382670.1 (A23-Q695)

301242  [NCBI]

AIVFIKEPSSQDALQGRRALLRCEVEAPDPVHVYWLLNGVPVQDTERRFAQGSSLSFAAVDRLQDSGAFQCVARDNITGEEARSANASFNIKWIEAGPVVLKHPASAAEIQPQTQVTLRCHIDGHPRPTYQWFRDGTPLSDDQSTHTVSSKERNLTLRPASPEHSGLYSCCAHNAFGQACSSQNFTLSIADESFARVLLAPQDVVVARNEEAMFHCQFSAQPPPSLQWVFEDETPITNRSRPPHLRKAMVFANGSLLLTQVRPRNAGVYRCIGQGQRGPPIVLEATLHLAEIEDMLPLEPRVFIAGGEERVACPAPQGLPTPSVWWEHAGVRLPAHGRVHQKGLELVFATIAESDAGVYTCHAANLAGQRRQDVNITVATVPTWLRKPQDSQLEEGKPGYLHCLTQATPKPTVIWYRNQMLISEDSRFEVSKNGTLRINSVEVYDGTVYRCVSSTPAGSIEAQARVQVLEKLKFTPPPQPQQCMEFDKEATVPCSATGREKPTVKWVRADGSSLPEWVTDNAGTLHFARVTRDDAGNYTCIASNEPQGQIRAHVQLTVAVFITFKVEPERTTVYQGHTALLRCEAQGDPKPLIQWKGKDRILDPTKLGPRMHIFQNGSLVIHDVAPEDSGSYTCIAGNSCNIRHTEAPLLVVDKPLMEDSEGPGSPPPYKMIQ

95-130 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.