Surfen dihydrochloride  (Synonyms: Aminoquinuride dihydrochloride)

Surfen dihydrochloride is a potent heparan sulfate antagonist. Surfen inhibits FGF2 binding and signal transduction. Surfen binds to glycosaminoglycans and reduces tau hyperphosphorylation. Surfen dihydrochloride inhibits the activity of recombinant uronyl 2-O-sulfotransferase with an IC₅₀ of approximately 2 μM. Surfen dihydrochloride inhibits HSV-1 viral infection. Surfen dihydrochloride inhibits neural differentiation, delays remyelination, and alleviates EAE^{[1][2][3][4][5][6]}.

Surfen (3 μM) dihydrochloride 可与 glycosaminoglycans (GAG) 结合^[1]。
Surfen (1 mg) dihydrochloride 可中和普通肝素和低分子量肝素的抗凝活性^[1]。
Surfen dihydrochloride 可抑制重组 uronyl 2-O-sulfotransferase 的活性，IC₅₀ 约为 2 μM^[1]。
Surfen (1-≥5 μM) dihydrochloride 以剂量依赖性方式抑制 CHO 细胞附着，并完全抑制 CHO 细胞中的 HSV-1 感染^[1]。
Surfen (5 μM) dihydrochloride 可有效抑制神经分化，并促进小鼠胚胎干细胞 (mESC) 多能性的维持^[2]。
Surfen (2.5-20 μM) dihydrochloride 可降低用抗 CD3/CD28 抗体包被的 T 细胞扩增珠激活的小鼠 T 细胞的增殖^[6]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Surfen (3 μM；2 天) dihydrochloride 在斑马鱼 tau 蛋白病变的 Tg[HuC::hTau^P301L; DsRed] 胚胎模型中可显著降低 tau 过度磷酸化，挽救脊髓运动神经元轴突分支缺陷和行为缺陷^[4]。
Surfen (5 mg/kg；腹腔注射；隔日一次) dihydrochloride 可缓解小鼠实验性自身免疫性脑脊髓炎 (EAE) 多发性硬化症模型中的临床症状，并减少 CD4 阳性 T 细胞和巨噬细胞向中枢神经系统的浸润^[5]。
Surfen (100 μM；注射至右侧钻孔) dihydrochloride 可延缓 Lysolecithin (LPC) 诱导的脱髓鞘小鼠模型中病变部位的髓鞘再生^[5]。
Surfen (20 mg/kg；腹腔注射；每日一次；连续 3 天) dihydrochloride 在小鼠中可抑制抗 CD3 抗体激活的 T 细胞增殖^[6]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

445.34

C₂₁H₂₂Cl₂N₆O

5424-37-3

固体

Off-white to gray

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

• [1]. Schuksz M, et al. Surfen, a small molecule antagonist of heparan sulfate. Proc Natl Acad Sci U S A. 2008 Sep 2;105(35):13075-80.  [Content Brief]

  [2]. Huang ML, et al. Small Molecule Antagonist of Cell Surface Glycosaminoglycans Restricts Mouse Embryonic Stem Cells in a Pluripotent State. Stem Cells. 2018 Jan;36(1):45-54.  [Content Brief]

  [3]. Varatharajan A, et al. Visual determination of heparin in serum utilizing surfen-induced aggregation emission enhancement of gold nanoclusters and heparin-induced fluorescence enhancement of surfen. Spectrochim Acta A Mol Biomol Spectrosc. 2025 Apr 15;339:126251.  [Content Brief]

  [4]. Alavi Naini SM, et al. Surfen and oxalyl surfen decrease tau hyperphosphorylation and mitigate neuron deficits in vivo in a zebrafish model of tauopathy. Transl Neurodegener. 2018 Mar 16;7:6.  [Content Brief]

  [5]. Warford JR, et al. Surfen, a proteoglycan binding agent, reduces inflammation but inhibits remyelination in murine models of Multiple Sclerosis. Acta Neuropathol Commun. 2018 Jan 4;6(1):4.  [Content Brief]

  [6]. Warford J, et al. Murine T cell activation is regulated by surfen (bis-2-methyl-4-amino-quinolyl-6-carbamide). Biochem Biophys Res Commun. 2014 Jan 10;443(2):524-30.  [Content Brief]