1. Epigenetics Anti-infection
  2. Histone Acetyltransferase Epigenetic Reader Domain Bacterial
  3. Anacardic Acid

Anacardic Acid  (Synonyms: 漆树酸; Hydroginkgolic acid; Ginkgolic Acid C15:0)

目录号: HY-N2020 纯度: 98.86%
COA 产品使用指南

Anacardic Acid 是从从腰果壳中提取液中分离到的酸类物质,为 histone acetyltransferases 抑制剂,对 p300 和 PCAF 的 IC50 值分别为 ∼8.5 μM 和 ∼5 μM。

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Anacardic Acid Chemical Structure

Anacardic Acid Chemical Structure

CAS No. : 16611-84-0

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥550
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5 mg ¥476
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10 mg ¥746
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25 mg ¥1534
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50 mg ¥2301
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100 mg ¥3451
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500 mg   询价  

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Top Publications Citing Use of Products
  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

Anacardic Acid, extracted from cashew nut shell liquid, is a histone acetyltransferase inhibitor, inhibits HAT activity of p300 and PCAF, with IC50s of ∼8.5 μM and ∼5 μM, respectively.

IC50 & Target[1]

p300-HAT

8.5 μM (IC50)

PCAF

5 μM (IC50)

体外研究
(In Vitro)

Anacardic Acid 是一种组蛋白乙酰转移酶,抑制 p300 和 PCAF 的 HAT 活性,IC50 分别为 ~8.5 μM 和 ~5 μM[1]。Anacardic Acid (300 μM) 抑制菌丝生长。Anacardic Acid (50 μM) 在 M 中诱导细胞凋亡样特征。oryzae,其作用与 caspase 无关。Anacardic Acid (1 -80 μM) 导致线粒体潜能丧失。Anacardic Acid (1-60 μM) 在 M. oryzae 表现出抗氧化活性。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Anacardic acid (5 mg/kg,ip) 减弱 HAT 与 MEF2A 启动子的结合,并逆转 C57BL/6 小鼠中去氧肾上腺素引起的 H3K9ac 过度乙酰化。Anacardic Acid 抑制 MEF2A 和心脏发育相关下游基因的转录水平,减弱由去氧肾上腺素引起的心脏下游基因的蛋白质过度表达,逆转和减弱暴露于去氧肾上腺素的小鼠心脏的心肌肥厚,并减弱左心室压力和改善心脏肥大小鼠的心脏功能[2]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

348.52

Formula

C22H36O3

CAS 号
性状

固体

颜色

White to off-white

中文名称

漆树酸

结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO 中的溶解度 : 100 mg/mL (286.93 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.8693 mL 14.3464 mL 28.6928 mL
5 mM 0.5739 mL 2.8693 mL 5.7386 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

In Vivo:

请根据您的 实验动物和给药方式 选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 方案 一

    请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 10 mg/mL (28.69 mM); 悬浊液; 超声助溶

    此方案可获得 10 mg/mL的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    1 mL 工作液为例,取 100 μL 100.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中,混合均匀。

    20% SBE-β-CD in Saline 的配制(4°C,储存一周):2 g SBE-β-CD(磺丁基醚 β-环糊精)粉末定容于 10 mL 的生理盐水中,完全溶解至澄清透明。
  • 方案 二

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.5 mg/mL (7.17 mM); 悬浊液; 超声助溶

    此方案可获得 2.5 mg/mL的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;再向上述体系中加入 50 μL Tween-80,混合均匀;然后再继续加入 450 μL 生理盐水 定容至 1 mL

    生理盐水的配制:将 0.9 g 氯化钠,溶解于 ddH₂O 并定容至 100 mL,可以得到澄清透明的生理盐水溶液。
动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
请输入您的动物体内配方组成:
%
DMSO +
+
%
Tween-80 +
%
Saline
如果您的动物是免疫缺陷鼠或者体弱鼠,建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
方案所需 助溶剂 包括:DMSO ,均可在 MCE 网站选购。 Tween 80,均可在 MCE 网站选购。
计算结果
工作液所需浓度 : mg/mL
储备液配制方法 : mg 药物溶于 μL  DMSO(母液浓度为 mg/mL)。
您所需的储备液浓度超过该产品的实测溶解度,以下方案仅供参考,如有需要,请与 MCE 中国技术支持联系。
动物实验体内工作液的配制方法 : 取 μL DMSO 储备液,加入 μL  μL ,混合均匀至澄清,再加 μL Tween 80,混合均匀至澄清,再加 μL 生理盐水
连续给药周期超过半月以上,请谨慎选择该方案。
请确保第一步储备液溶解至澄清状态,从左到右依次添加助溶剂。您可采用超声加热 (超声清洗仪,建议频次 20-40 kHz),涡旋吹打等方式辅助溶解。
纯度 & 产品资料

纯度: 98.86%

参考文献
Kinase Assay
[1]

Briefly, indicated amounts of proteins/peptide are incubated in HAT assay buffer containing 50 mM Tris-HCl, pH 8.0, 10% (v/v) glycerol, 1 mM dithiothreitol, 1 mM phenylmethyl sulfonyl fluoride, 0.1 mM EDTA, pH 8.0, 10 mM sodium butyrate at 30°C for 10 min in the presence or absence of compound followed by the addition of 1 μL of 6.2 Ci/mmol [3H]acetyl coenzyme A (acetyl-CoA) and are further incubated for another 10 min. The final reaction volume is 30 μL. The reaction mixture is then blotted onto P-81 filter papers, and radioactive counts are recorded on a Wallac 1409 liquid scintillation counter. To characterize the inhibition kinetics of anacardic acid, filter binding assays are done using a constant amount of HeLa core histones in the presence or absence of AA with increasing concentrations of [3H]acetyl-CoA[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Assay
[3]

Mycelial cell death assay is performed to evaluate the number of colony-forming units in treated and untreated samples. M. oryzae conidia (106 conidia/mL) are allowed to germinate in 100-mL flasks with 20 mL complete medium broth (CM) at 28°C in a rotary shaker (200 rpm) for 12 h. The cultures are exposed to different concentrations of anacardic acid for 2 h. The germinated conidia are washed with sterile water, diluted to 104 conidia/mL, and plated on oat meal agar and incubated at 28°C for 3 days. Colony-forming units (CFUs) are counted in each of the three ndividual experiments performed, and values are plotted in the graph as average of three replicates. The data in each sample is expressed as the percentage of the total number of CFUs observed in untreated or 0.1 % DMSO treated control[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[2]

Pathogen-free male and female 11-13 week-old C57BL/6 mice (18-20 g) are randomly selected to inject phenylephrine (20 mg/kg) (control groups receive equivalent normal saline). In some cases, phenylephrine-treated C57BL/6 mice are administered with a Chinese herbal extract anacardic acid (5 mg/kg). Anacardic acid is dissolved in sterile DMSO at a concentration of 1 mg/ml and stored at 4°C. Phenylephrine is administered by a subcutaneous injection at a dose of 20 mg per kg per day continuously for 30 days. Moreover, anacardic acid is administered by an intraperitoneal injection at a dose of 5 mg/kg every 3rd day intraperitoneal injection at a dose of 5 mg/kg every 3rd day. After modeling, mice are euthanized using 20% carbon dioxide in an anesthesia chamber until they are unresponsive to nose pinch and the hearts are isolated[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.8693 mL 14.3464 mL 28.6928 mL 71.7319 mL
5 mM 0.5739 mL 2.8693 mL 5.7386 mL 14.3464 mL
10 mM 0.2869 mL 1.4346 mL 2.8693 mL 7.1732 mL
15 mM 0.1913 mL 0.9564 mL 1.9129 mL 4.7821 mL
20 mM 0.1435 mL 0.7173 mL 1.4346 mL 3.5866 mL
25 mM 0.1148 mL 0.5739 mL 1.1477 mL 2.8693 mL
30 mM 0.0956 mL 0.4782 mL 0.9564 mL 2.3911 mL
40 mM 0.0717 mL 0.3587 mL 0.7173 mL 1.7933 mL
50 mM 0.0574 mL 0.2869 mL 0.5739 mL 1.4346 mL
60 mM 0.0478 mL 0.2391 mL 0.4782 mL 1.1955 mL
80 mM 0.0359 mL 0.1793 mL 0.3587 mL 0.8966 mL
100 mM 0.0287 mL 0.1435 mL 0.2869 mL 0.7173 mL
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    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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HY-N2020
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